Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade

Abstract

The paper covers investigations of stray dogs in the territory of the city of Belgrade. A total of 184 blood serum samples were examined for the presence of antibodies specific to Brucella canis. The method of slow agglutination in a test tube with 2- mercaptoethanol was used in the diagnostic procedure. Of the 184 examined serums, 49 (26.63%) had a titer of 1/50, 25 serums had a titer of 1/100 (13.58%), while 20 serums had a titer equal to or bigger than 1/200 (10.87%). Furthermore, 15 samples of full blood from serologiclly negative animals were also presented for isolation. The bacteriological finding for these samples was negative. The obtained results indicate that the number of seropositive stray dogs in the territory of Belgrade is extremely high and that 10.87% of the testes animals are definitely infected with Brucella canis.

Ovim radom obuhvaćeno je ispitivanje pasa lutalica na teritoriji grada Beograda. Na prisustvo antitela specifičnih na Brucella canis ukupno je ispitano 184 uzorka krvnih seruma. U dijagnostikovanju se koristila metoda spore aglutinacije u epruveti sa 2-merkaptoetanolom. Od 184 ispitana seruma, 49 (26,63%) seruma je imalo titar 1/50, 25 seruma je imalo titar 1/100 (13,58%), dok je 20 seruma imalo titar jednak ili veći od 1/200 (10,87%). Za izolaciju B. canis je dostavljeno i 15 uzoraka pune krvi od serološki negativnih životinja. Bakteriološki nalaz ovih uzoraka je bio negativan. Dobijeni rezultati ukazuju da je broj sero-pozitivnih pasa lutalica na teritoriji Beograda izuzetno visok i da je 10,87 posto testiranih pasa sigurno inficirano bakterijom Brucella canis.