Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo
Evaluacija genotoksičnog potencijala amitraza citogenetičkim testom in vivo
Апстракт
The genotoxic effect of amitraz (3.6 mg/kg b.m, 1.8 mg/kg b.m, 0.9 mg/kg b.m) on bone marrow cells of HAN strain mice was examined in vivo by following the frequency of numeric and structural chromosomal aberrations, while the cytostatic effect of the same preparation was followed through the mitotic index. The first experimental dose (3.6 mg/kg b.m) caused a statistically significant (p lt 0.01) increase in the mitotic index, while the second (1.8 mg/kg b.m) and the third (0.9 mg/kg b.m.) experimental dose statistically significantly (p lt 0.01) decreased the value of the mitotic index in animals of both sexes. All the applied doses exhibited an ability to induce numeric (aneuploidy and polyploidy) and structural (Robertsonian translocation) chromosomal aberrations. It can be concluded on the basis of the obtained results that the examined substance exhibits a genotoxic dose-dependant effect. .
Obavljena su ispitivanja genotoksičnog efekta amitraza (3,6 mg/kg t.m, 1.8 mg/kg t.m, 0.9 mg/kg t.m) na ćelijama kostne srži miša HAN soja in vivo, praćenjem učestalosti numeričkih i strukturnih hromozomskih aberacija, dok je citostatski efekat istog preparata praćen preko mitotskog indeksa. Prva eksperimentalna doza (3,6 mg/kg t.m) uslovila je statistički značajno (p lt 0,01) povećanje mitotskog indeksa, dok su druga (1,8 mg/kg t.m) i treća (0,9 mg/kg t.m) eksperimentalna doza statistički značajno (p lt 0,01) smanjile vrednost mitotskog indeksa kod jedinki oba pola. Sve primenjene doze pokazale su sposobnost indukcije numeričkih (aneuploidije i poliploidije) i strukturnih (Robertsonove translokacije) hromozomskih aberacija. Na osnovu dobijenih rezultata može da se zaključi da ispitivana supstancija pokazuje genotoksični dozno zavisni efekat. .
Кључне речи:
amitraz / genotoxicity / cytostatic effect / mitotic index / structural aberrations / numeric aberrations / amitraz / genotoksičnost / citostatski efekat / mitotskiindeks / strukturne aberacije / numeričke aberacijeИзвор:
Veterinarski Glasnik, 2006, 60, 3-4, 163-173Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Pejin, Ivana I. AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Kulišić, Zoran PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/393 AB - The genotoxic effect of amitraz (3.6 mg/kg b.m, 1.8 mg/kg b.m, 0.9 mg/kg b.m) on bone marrow cells of HAN strain mice was examined in vivo by following the frequency of numeric and structural chromosomal aberrations, while the cytostatic effect of the same preparation was followed through the mitotic index. The first experimental dose (3.6 mg/kg b.m) caused a statistically significant (p lt 0.01) increase in the mitotic index, while the second (1.8 mg/kg b.m) and the third (0.9 mg/kg b.m.) experimental dose statistically significantly (p lt 0.01) decreased the value of the mitotic index in animals of both sexes. All the applied doses exhibited an ability to induce numeric (aneuploidy and polyploidy) and structural (Robertsonian translocation) chromosomal aberrations. It can be concluded on the basis of the obtained results that the examined substance exhibits a genotoxic dose-dependant effect. . AB - Obavljena su ispitivanja genotoksičnog efekta amitraza (3,6 mg/kg t.m, 1.8 mg/kg t.m, 0.9 mg/kg t.m) na ćelijama kostne srži miša HAN soja in vivo, praćenjem učestalosti numeričkih i strukturnih hromozomskih aberacija, dok je citostatski efekat istog preparata praćen preko mitotskog indeksa. Prva eksperimentalna doza (3,6 mg/kg t.m) uslovila je statistički značajno (p lt 0,01) povećanje mitotskog indeksa, dok su druga (1,8 mg/kg t.m) i treća (0,9 mg/kg t.m) eksperimentalna doza statistički značajno (p lt 0,01) smanjile vrednost mitotskog indeksa kod jedinki oba pola. Sve primenjene doze pokazale su sposobnost indukcije numeričkih (aneuploidije i poliploidije) i strukturnih (Robertsonove translokacije) hromozomskih aberacija. Na osnovu dobijenih rezultata može da se zaključi da ispitivana supstancija pokazuje genotoksični dozno zavisni efekat. . PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo T1 - Evaluacija genotoksičnog potencijala amitraza citogenetičkim testom in vivo VL - 60 IS - 3-4 SP - 163 EP - 173 DO - 10.2298/VETGL0604163P ER -
@article{ author = "Pejin, Ivana I. and Stanimirović, Zoran and Stevanović, Jevrosima and Kulišić, Zoran", year = "2006", abstract = "The genotoxic effect of amitraz (3.6 mg/kg b.m, 1.8 mg/kg b.m, 0.9 mg/kg b.m) on bone marrow cells of HAN strain mice was examined in vivo by following the frequency of numeric and structural chromosomal aberrations, while the cytostatic effect of the same preparation was followed through the mitotic index. The first experimental dose (3.6 mg/kg b.m) caused a statistically significant (p lt 0.01) increase in the mitotic index, while the second (1.8 mg/kg b.m) and the third (0.9 mg/kg b.m.) experimental dose statistically significantly (p lt 0.01) decreased the value of the mitotic index in animals of both sexes. All the applied doses exhibited an ability to induce numeric (aneuploidy and polyploidy) and structural (Robertsonian translocation) chromosomal aberrations. It can be concluded on the basis of the obtained results that the examined substance exhibits a genotoxic dose-dependant effect. ., Obavljena su ispitivanja genotoksičnog efekta amitraza (3,6 mg/kg t.m, 1.8 mg/kg t.m, 0.9 mg/kg t.m) na ćelijama kostne srži miša HAN soja in vivo, praćenjem učestalosti numeričkih i strukturnih hromozomskih aberacija, dok je citostatski efekat istog preparata praćen preko mitotskog indeksa. Prva eksperimentalna doza (3,6 mg/kg t.m) uslovila je statistički značajno (p lt 0,01) povećanje mitotskog indeksa, dok su druga (1,8 mg/kg t.m) i treća (0,9 mg/kg t.m) eksperimentalna doza statistički značajno (p lt 0,01) smanjile vrednost mitotskog indeksa kod jedinki oba pola. Sve primenjene doze pokazale su sposobnost indukcije numeričkih (aneuploidije i poliploidije) i strukturnih (Robertsonove translokacije) hromozomskih aberacija. Na osnovu dobijenih rezultata može da se zaključi da ispitivana supstancija pokazuje genotoksični dozno zavisni efekat. .", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo, Evaluacija genotoksičnog potencijala amitraza citogenetičkim testom in vivo", volume = "60", number = "3-4", pages = "163-173", doi = "10.2298/VETGL0604163P" }
Pejin, I. I., Stanimirović, Z., Stevanović, J.,& Kulišić, Z.. (2006). Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(3-4), 163-173. https://doi.org/10.2298/VETGL0604163P
Pejin II, Stanimirović Z, Stevanović J, Kulišić Z. Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo. in Veterinarski Glasnik. 2006;60(3-4):163-173. doi:10.2298/VETGL0604163P .
Pejin, Ivana I., Stanimirović, Zoran, Stevanović, Jevrosima, Kulišić, Zoran, "Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo" in Veterinarski Glasnik, 60, no. 3-4 (2006):163-173, https://doi.org/10.2298/VETGL0604163P . .