Evaluation of genotoxic potential of amitraz by cytogenetic test in vivo

Abstract

The genotoxic effect of amitraz (3.6 mg/kg b.m, 1.8 mg/kg b.m, 0.9 mg/kg b.m) on bone marrow cells of HAN strain mice was examined in vivo by following the frequency of numeric and structural chromosomal aberrations, while the cytostatic effect of the same preparation was followed through the mitotic index. The first experimental dose (3.6 mg/kg b.m) caused a statistically significant (p lt 0.01) increase in the mitotic index, while the second (1.8 mg/kg b.m) and the third (0.9 mg/kg b.m.) experimental dose statistically significantly (p lt 0.01) decreased the value of the mitotic index in animals of both sexes. All the applied doses exhibited an ability to induce numeric (aneuploidy and polyploidy) and structural (Robertsonian translocation) chromosomal aberrations. It can be concluded on the basis of the obtained results that the examined substance exhibits a genotoxic dose-dependant effect. .

Obavljena su ispitivanja genotoksičnog efekta amitraza (3,6 mg/kg t.m, 1.8 mg/kg t.m, 0.9 mg/kg t.m) na ćelijama kostne srži miša HAN soja in vivo, praćenjem učestalosti numeričkih i strukturnih hromozomskih aberacija, dok je citostatski efekat istog preparata praćen preko mitotskog indeksa. Prva eksperimentalna doza (3,6 mg/kg t.m) uslovila je statistički značajno (p lt 0,01) povećanje mitotskog indeksa, dok su druga (1,8 mg/kg t.m) i treća (0,9 mg/kg t.m) eksperimentalna doza statistički značajno (p lt 0,01) smanjile vrednost mitotskog indeksa kod jedinki oba pola. Sve primenjene doze pokazale su sposobnost indukcije numeričkih (aneuploidije i poliploidije) i strukturnih (Robertsonove translokacije) hromozomskih aberacija. Na osnovu dobijenih rezultata može da se zaključi da ispitivana supstancija pokazuje genotoksični dozno zavisni efekat. .