Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens
Rezidue enrofloksacina i njegovog glavnog metabolita ciprofloksacina kod brojlera
Apstrakt
The presence of enrofloxacin residues in muscle and liver after peroral administration of enrofloxacin (10 mg/kg bw/day) to chickens was studied. Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 and HPLC with fluorescence detection were used for the detection of enrofloxacin residues. During the 5 days dosing period, enrofloxacin concentrations in breast muscle and liver greatly exceeded the EU MRL values. Ciprofloxacin was not detected in muscles after 2 days of dosing, but it was detected in the liver in concentrations over the MRL. During the post treatment period enrofloxacin and ciprofloxacin concentrations in the breast muscle and liver exceeded the MRL values until the 4th day of the withdrawal period. Ciprofloxacin was not detected in the breast muscle after 4 days post dosing, but it was detected in the liver at concentrations below MRL. Four days withdrawal period is the allowed time for the drug concentration to decrease to an acceptable level in the m...eat and liver, prior to slaughter (below MRL). Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 gave positive results in all the samples which had residues above MRL values.
Prisustvo rezidua enrofloksacina u mesu i jetri pilića ispitivano je posle peroralnog aplikovanja enrofloksacina (10 mg/kg t.m./dan. Za ispitivanje rezidua su korišćene: mikrobiološka metoda - ploča pH 8.0 sa Escherichia coli NCIMB 11595 i HPLC metoda sa fluorescentnom detekcijom. Tokom pet dana primene terapije, koncentracije enrofloksacina u grudnoj muskulaturi i jetri su bile značajno iznad EU maksimalno dozvoljenih koncentracija (MDK). Ciprofloksacin nije detektovan u muskulaturi drugog dana primene terapije ali je detektovan u jetri u koncentracijama iznad MDK. Posle prekida terapije, koncentracije enrofloksacina i ciprofloksacina u grudnoj muskulaturi i jetri su bile iznad MDK nivoa do četvrtog dana karence. Ciprofloksacin nije detektovan u muskulaturi četvrtog dana karence ali je detektovan u jetri u koncentracijama ispod MDK. Četiri dana karence su period koji omogućuje da koncentracija leka u mesu i jetri padne na prihvatljiv nivo pre klanja (ispod MDK). Mikrobiološka metoda -... ploča pH 8.0 sa Escherichia coli NCIMB 11595 daje pozitivne rezultate u svim uzorcima u kojima je sadržaj rezidua bio iznad MDK.
Ključne reči:
chickens / enrofloxacin / HPLC / microbiologicalmethod / residuesIzvor:
Acta Veterinaria-Beograd, 2006, 56, 5-6, 497-506Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0606497P
ISSN: 0567-8315
WoS: 000242961900013
Scopus: 2-s2.0-33846459641
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Petrović, Jelena AU - Baltić, Milan Ž. AU - Ćupić, Vitomir AU - Stefanović, S. AU - Stojanović, Dragica PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/419 AB - The presence of enrofloxacin residues in muscle and liver after peroral administration of enrofloxacin (10 mg/kg bw/day) to chickens was studied. Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 and HPLC with fluorescence detection were used for the detection of enrofloxacin residues. During the 5 days dosing period, enrofloxacin concentrations in breast muscle and liver greatly exceeded the EU MRL values. Ciprofloxacin was not detected in muscles after 2 days of dosing, but it was detected in the liver in concentrations over the MRL. During the post treatment period enrofloxacin and ciprofloxacin concentrations in the breast muscle and liver exceeded the MRL values until the 4th day of the withdrawal period. Ciprofloxacin was not detected in the breast muscle after 4 days post dosing, but it was detected in the liver at concentrations below MRL. Four days withdrawal period is the allowed time for the drug concentration to decrease to an acceptable level in the meat and liver, prior to slaughter (below MRL). Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 gave positive results in all the samples which had residues above MRL values. AB - Prisustvo rezidua enrofloksacina u mesu i jetri pilića ispitivano je posle peroralnog aplikovanja enrofloksacina (10 mg/kg t.m./dan. Za ispitivanje rezidua su korišćene: mikrobiološka metoda - ploča pH 8.0 sa Escherichia coli NCIMB 11595 i HPLC metoda sa fluorescentnom detekcijom. Tokom pet dana primene terapije, koncentracije enrofloksacina u grudnoj muskulaturi i jetri su bile značajno iznad EU maksimalno dozvoljenih koncentracija (MDK). Ciprofloksacin nije detektovan u muskulaturi drugog dana primene terapije ali je detektovan u jetri u koncentracijama iznad MDK. Posle prekida terapije, koncentracije enrofloksacina i ciprofloksacina u grudnoj muskulaturi i jetri su bile iznad MDK nivoa do četvrtog dana karence. Ciprofloksacin nije detektovan u muskulaturi četvrtog dana karence ali je detektovan u jetri u koncentracijama ispod MDK. Četiri dana karence su period koji omogućuje da koncentracija leka u mesu i jetri padne na prihvatljiv nivo pre klanja (ispod MDK). Mikrobiološka metoda - ploča pH 8.0 sa Escherichia coli NCIMB 11595 daje pozitivne rezultate u svim uzorcima u kojima je sadržaj rezidua bio iznad MDK. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens T1 - Rezidue enrofloksacina i njegovog glavnog metabolita ciprofloksacina kod brojlera VL - 56 IS - 5-6 SP - 497 EP - 506 DO - 10.2298/AVB0606497P ER -
@article{ author = "Petrović, Jelena and Baltić, Milan Ž. and Ćupić, Vitomir and Stefanović, S. and Stojanović, Dragica", year = "2006", abstract = "The presence of enrofloxacin residues in muscle and liver after peroral administration of enrofloxacin (10 mg/kg bw/day) to chickens was studied. Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 and HPLC with fluorescence detection were used for the detection of enrofloxacin residues. During the 5 days dosing period, enrofloxacin concentrations in breast muscle and liver greatly exceeded the EU MRL values. Ciprofloxacin was not detected in muscles after 2 days of dosing, but it was detected in the liver in concentrations over the MRL. During the post treatment period enrofloxacin and ciprofloxacin concentrations in the breast muscle and liver exceeded the MRL values until the 4th day of the withdrawal period. Ciprofloxacin was not detected in the breast muscle after 4 days post dosing, but it was detected in the liver at concentrations below MRL. Four days withdrawal period is the allowed time for the drug concentration to decrease to an acceptable level in the meat and liver, prior to slaughter (below MRL). Microbiological method - plate pH 8.0 with Escherichia coli NCIMB 11595 gave positive results in all the samples which had residues above MRL values., Prisustvo rezidua enrofloksacina u mesu i jetri pilića ispitivano je posle peroralnog aplikovanja enrofloksacina (10 mg/kg t.m./dan. Za ispitivanje rezidua su korišćene: mikrobiološka metoda - ploča pH 8.0 sa Escherichia coli NCIMB 11595 i HPLC metoda sa fluorescentnom detekcijom. Tokom pet dana primene terapije, koncentracije enrofloksacina u grudnoj muskulaturi i jetri su bile značajno iznad EU maksimalno dozvoljenih koncentracija (MDK). Ciprofloksacin nije detektovan u muskulaturi drugog dana primene terapije ali je detektovan u jetri u koncentracijama iznad MDK. Posle prekida terapije, koncentracije enrofloksacina i ciprofloksacina u grudnoj muskulaturi i jetri su bile iznad MDK nivoa do četvrtog dana karence. Ciprofloksacin nije detektovan u muskulaturi četvrtog dana karence ali je detektovan u jetri u koncentracijama ispod MDK. Četiri dana karence su period koji omogućuje da koncentracija leka u mesu i jetri padne na prihvatljiv nivo pre klanja (ispod MDK). Mikrobiološka metoda - ploča pH 8.0 sa Escherichia coli NCIMB 11595 daje pozitivne rezultate u svim uzorcima u kojima je sadržaj rezidua bio iznad MDK.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens, Rezidue enrofloksacina i njegovog glavnog metabolita ciprofloksacina kod brojlera", volume = "56", number = "5-6", pages = "497-506", doi = "10.2298/AVB0606497P" }
Petrović, J., Baltić, M. Ž., Ćupić, V., Stefanović, S.,& Stojanović, D.. (2006). Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(5-6), 497-506. https://doi.org/10.2298/AVB0606497P
Petrović J, Baltić MŽ, Ćupić V, Stefanović S, Stojanović D. Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens. in Acta Veterinaria-Beograd. 2006;56(5-6):497-506. doi:10.2298/AVB0606497P .
Petrović, Jelena, Baltić, Milan Ž., Ćupić, Vitomir, Stefanović, S., Stojanović, Dragica, "Residues of enrofloxacin and its main metabolite ciprofloxacin in broiler chickens" in Acta Veterinaria-Beograd, 56, no. 5-6 (2006):497-506, https://doi.org/10.2298/AVB0606497P . .