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Efekti aspirina na apoptozu neutrofilnih granulocita

dc.creatorVasilev, Saša
dc.creatorMajstorović, Ivana
dc.creatorGašić, Sonja
dc.creatorVučević, Dragana
dc.creatorVasilijić, S.
dc.creatorĆupić, Vitomir
dc.creatorČolić, M.
dc.date.accessioned2020-06-03T13:01:00Z
dc.date.available2020-06-03T13:01:00Z
dc.date.issued2006
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/422
dc.description.abstractNeutrophils are a part of the immune system, and they are involved in host defence against microorganisms. Neutrophil granulocytes have the shortest lifespan among leukocytes, which can be modulated by cytokines and pharmacological agents. The effect of aspirin on apoptosis of inflammatory granulocytes has not been studied in detail yet, and therefore was the chosen subject of this study. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were more than 95% neutrophil granulocytes. The cells were cultivated 24 h with different concentrations of aspirin ranging from 1 µM to 10 mM. After the cultivation period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that at concentrations from 0,1 mM to 2,5 mM aspirin inhibited apoptosis of granulocytes, but at 10 mM aspirin induced apoptosis of these cells.en
dc.description.abstractNeutrofilni granulociti su deo imunog sistema, uključenog u odbranu od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima, a do sada nije ispitivan efekat aspirina na apoptozu inflamatornih granulocita. Zbog toga je u ovoj studiji ispitivan efekat aspirina na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera, potkožno implantiranih, pacovima Albino Oxford (AO) soja. Inflamatorne ćelije, izolovane 20 sati kasnije, najvećim delom (više od 95 %) predstavljaju neutrofilne granulocite. Ove ćelije su kultivisane 24 sata sa aspirinom u koncentracijama od 1 µM do 10 mM. Posle ovog perioda supernatanti su sakupljani i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovljeno je da u koncentracijama od 0,1 do 2,5 mM aspirin inhibira, a samo u visokim koncentracijama (10 mM) indukuje apoptozu ovih ćelija. Aspirinom indukovana apoptoza je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectaspirinen
dc.subjectneutrophil granulocytesen
dc.subjectapoptosisen
dc.titleThe effects of aspirin on apoptosis of neutrophil granulocytesen
dc.titleEfekti aspirina na apoptozu neutrofilnih granulocitasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЋупић, Витомир; Василев, Саша; Мајсторовић, Ивана; Гашић, Соња; Вучевић, Драгана; Василијић, С.; Чолић, М.; Ефекти аспирина на апоптозу неутрофилних гранулоцита; Ефекти аспирина на апоптозу неутрофилних гранулоцита;
dc.citation.volume56
dc.citation.issue5-6
dc.citation.spage413
dc.citation.epage421
dc.citation.other56(5-6): 413-421
dc.citation.rankM23
dc.identifier.wos000242961900004
dc.identifier.doi10.2298/AVB0606413V
dc.identifier.scopus2-s2.0-33846437850
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/987/421.pdf
dc.identifier.rcubconv_645
dc.type.versionpublishedVersion


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