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dc.creatorStanimirović, Zoran
dc.creatorStevanović, Jevrosima
dc.creatorBajić, Vladan
dc.creatorRadović, Ivica
dc.date.accessioned2020-06-03T13:02:27Z
dc.date.available2020-06-03T13:02:27Z
dc.date.issued2007
dc.identifier.issn1383-5718
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/443
dc.description.abstractFumagillin is a naturally secreted antibiotic of the fungus Aspergillus fumigates. It is used in veterinary medicine against microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin (in the form of fumagillin dicyclohexylamine) was evaluated in mouse bone-marrow cells using the mitotic index (MI), the chromosome aberration (CA) assay, and the micronucleus (MN) test. Fumagillin was administered to BALB/c mice by gavage, at doses of 25, 50, 75 mg/kg body weight (bw), repeated for 7 days at 24-h intervals, with water-sugar syrup as a negative control and cyclophosphamide (40 mg/kg bw) as a positive control. All experimental doses of fumagillin induced a significant decrease (p < 0.001) in MI (3.47 +/- 0.04%, 3.17 +/- 0.01%, and 2.27 +/- 0.02%, respectively) in comparison with the negative control (6.00 +/- 0.01%). Fumagillin significantly (p < 0.001) increased the frequency of MN (4.98 +/- 0.35, 8.45 +/- 0.57, and 12.02 +/- 0.37, respectively) over negative control (1.04 +/- 0.28). Significantly increased frequencies (p < 0.01 or p < 0.001) of numerical chromosomal aberrations (aneuploidies and polyploidies) and structural chromosomal aberrations such as gaps, breaks, and centric rings were observed at the highest experimental dose of fumagillin (75 mg/kg bw) compared with the negative control. However, with respect to the induction of Robertsonian translocations, both the intermediate (50 mg/kg bw) and highest (75 mg/kg bw) experimental dose caused a significant (p < 0.001) increase (7.12 +/- 0.26 and 9.00 +/- 0.10, respectively) in comparison with the negative control (0.00 +/- 0.00). Chromosomes 4 and 19 participated in these Robertsonian translocations. Regarding total cytogenetic changes, a significant increase (p < 0.001) was observed in both the intermediate dose group (17.36 +/- 1.83) and the highest dose group (59.49 +/- 1.92) compared with the negative control (7.00 +/- 1.35). These results suggest that fumagillin has genotoxic (clastogenic) potential in mammals in vivo.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143022/RS//
dc.rightsrestrictedAccess
dc.sourceMutation Research-Genetic Toxicology and Environmental Mutagenesis
dc.subjectfumagillinen
dc.subjectgenotoxicityen
dc.subjectmitotic index (MI)en
dc.subjectmicronuclei (MN)en
dc.subjectchromosome aberrations (CA)en
dc.subjectRobertsonian chromosome Rb(4.19)en
dc.titleEvaluation of genotoxic effects of fumagillin by cytogenetic tests in vivoen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБајић, Владан; Радовић, Ивица; Станимировић, Зоран; Стевановић, Јевросима;
dc.citation.volume628
dc.citation.issue1
dc.citation.spage1
dc.citation.epage10
dc.citation.other628(1): 1-10
dc.citation.rankM22
dc.identifier.wos000245320900001
dc.identifier.doi10.1016/j.mrgentox.2006.09.014
dc.identifier.pmid17258933
dc.identifier.scopus2-s2.0-33847196764
dc.identifier.rcubconv_1537
dc.type.versionpublishedVersion


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