Show simple item record

dc.creatorKovačević-Filipović, Milica
dc.creatorPetakov, Marijana
dc.creatorHermitte, Francis
dc.creatorDebeissat, Christelle
dc.creatorKrstić, Aleksandra
dc.creatorJovčić, Gordana
dc.creatorBiligarski, Dijana
dc.creatorLafarge, Xavier
dc.creatorMilenković, Pavle
dc.creatorPraloran, Vincent
dc.creatorIvanović, Zoran
dc.date.accessioned2020-06-03T13:03:31Z
dc.date.available2020-06-03T13:03:31Z
dc.date.issued2007
dc.identifier.issn0021-9541
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/458
dc.description.abstractLow O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.en
dc.publisherWiley-Liss, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145048/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Cellular Physiology
dc.titleInterleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМиленковић, Павле; Хермитте, Францис; Ивановић, Зоран; Крстић, Aлександра; Дебеиссат, Цхристелле; Ковачевић-Филиповић, Милица; Пралоран, Винцент; Лафарге, Xавиер; Билигарски, Дијана; Петаков, Маријана; Јовчић, Гордана;
dc.citation.volume212
dc.citation.issue1
dc.citation.spage68
dc.citation.epage75
dc.citation.other212(1): 68-75
dc.citation.rankM21
dc.identifier.wos000246789300008
dc.identifier.doi10.1002/jcp.21003
dc.identifier.pmid17311290
dc.identifier.scopus2-s2.0-34249905641
dc.identifier.rcubconv_1546
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record