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Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro

dc.creatorNišavić, Jakov
dc.creatorMilić, Nenad
dc.creatorVeljović, Ljubiša
dc.date.accessioned2020-06-03T13:04:58Z
dc.date.available2020-06-03T13:04:58Z
dc.date.issued2007
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/479
dc.description.abstractThe objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera.en
dc.description.abstractCilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena njihova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectNewcastle disease virusen
dc.subjectfusion testen
dc.subjecthemagglutinationen
dc.subjecthemolysisen
dc.subjecthemadsorptionen
dc.titleExamination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitroen
dc.titleIspitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitrosr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВељовић, Љубиша; Нишавић, Јаков; Милић, Ненад;
dc.citation.volume57
dc.citation.issue1
dc.citation.spage3
dc.citation.epage10
dc.citation.other57(1): 3-10
dc.citation.rankM23
dc.identifier.wos000245603000001
dc.identifier.doi10.2298/AVB0701003N
dc.identifier.scopus2-s2.0-34247572339
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/1040/478.pdf
dc.identifier.rcubconv_651
dc.type.versionpublishedVersion


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