Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N = 4) and field isolates (N = 4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantific...ation of the pathogen.
Кључне речи:
American foulbrood / endospores / genotypes / germination / Paenibacillus larvaeИзвор:
Veterinary Microbiology, 2008, 129, 3-4, 342-349Издавач:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/j.vetmic.2007.12.001
ISSN: 0378-1135
PubMed: 18207338
WoS: 000256598700013
Scopus: 2-s2.0-43249121392
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Forsgren, Eva AU - Stevanović, Jevrosima AU - Fries, Ingemar PY - 2008 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/547 AB - There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N = 4) and field isolates (N = 4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantification of the pathogen. PB - Elsevier Science Bv, Amsterdam T2 - Veterinary Microbiology T1 - Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes VL - 129 IS - 3-4 SP - 342 EP - 349 DO - 10.1016/j.vetmic.2007.12.001 ER -
@article{ author = "Forsgren, Eva and Stevanović, Jevrosima and Fries, Ingemar", year = "2008", abstract = "There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N = 4) and field isolates (N = 4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantification of the pathogen.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Veterinary Microbiology", title = "Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes", volume = "129", number = "3-4", pages = "342-349", doi = "10.1016/j.vetmic.2007.12.001" }
Forsgren, E., Stevanović, J.,& Fries, I.. (2008). Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. in Veterinary Microbiology Elsevier Science Bv, Amsterdam., 129(3-4), 342-349. https://doi.org/10.1016/j.vetmic.2007.12.001
Forsgren E, Stevanović J, Fries I. Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. in Veterinary Microbiology. 2008;129(3-4):342-349. doi:10.1016/j.vetmic.2007.12.001 .
Forsgren, Eva, Stevanović, Jevrosima, Fries, Ingemar, "Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes" in Veterinary Microbiology, 129, no. 3-4 (2008):342-349, https://doi.org/10.1016/j.vetmic.2007.12.001 . .