Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite

Abstract

Sixty Holstein newborn calves, which were immediately after parturition separated from their dams and placed in individual pens, were randomly assigned to 4 treatment groups (15 calves per group). All calves were bottle-fed twice a day during the first 48 hours after partus, with their mothers' colostrum in 12 hour intervals, starting two hours after partus, according to the following model: i) group 1 (G1) received 0.75 L of colostrum (C) per meal, with 20 mL of 25% zeolite (clinoptilolite) suspension in distilled water; ii) group 2 (G2) received 1.5L of C per meal, with 20 mL of 25% zeolite suspension in distilled water; iii) group 3 (G3) received 0.75 L of C per meal, and iv) group 4 (G4) received 1.5 L of C per meal. Blood serum immunoglobulin G (IgG) concentrations at 0h (before C ingestion) 6h, 24h and 48h after partus and colostrum IgG concentrations were determined using commercial sRID plates (INEP, Zemun, Serbia). Apparent efficiency of absorption (AEA%) was calculated for individual calves at 6h after partus. Calf blood serum IgG concentrations at all investigated time intervals in G1 were increased compared to G3, with a significant increase at 6h after partus (15±4:10±5 g/L, p lt 0.05). The same relationship was evident between G2 and G4, with significant mean IgG blood serum differences evident at 6, 24. and 48. hour after partus. Calf blood serum IgG concentration was significantly higher in G2 compared to all experimental groups at 6, 24 and 48h after partus. Apparent efficiency of absorption (AEA%) at 6h after partus was significantly higher in G1, compared to all other experimental groups.

Šezdeset novorođenih teladi Holštajn rase, koja su neposredno nakon rođenja odvojena od majki i stavljena u individualne bokseve, su metodom slučajnog izbora podeljene u 4 ogledne grupe (15 teladi po grupi). Sva telad su za vreme prvih 48 sati života hranjena dva puta dnevno (u intervalu od 12 sati) kolostrumom majki, počevši od drugog sata nakon partusa prema sledećem eksperimentalnom modelu: i) grupa 1 (G1) dobijala je 0.75 L kolostruma (C) po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; ii) grupa 2 (G2) dobijala je 1.5 L C po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; iii) grupa 3 (G3) dobijala je 0.75L C po obroku; iv) grupa 4 (G4) dobijala je 1.5 L C po obroku. Koncentracija imunoglobulina G (IgG) određivana je komercijalnim radijalnim imunodifuzionim testom (sRID, INEP, Zemin) u krvnom serumu dobijenom iz uzoraka krvi uzetih punkcijom v. jugularis pre uzimanja kolosruma (0. sat), 6-og, 24-og i 48-og sata nakon rođenja, dok je relativna efikasnost apsorpcije imunoglobulina (AEA%) određena 6-og sata po rođenju. Koncentracija IgG u krvnom serumu kod teladi iz grupe G1 u odnosu na G2 bila je viša 6-og, 24-og i 48-og sata, dok je statistička značajnost razlika srednjih vrednosti ustanovljena 6-og sata po rođenju (15±4:10±5 g/L, p lt 0.05). Isti odnos srednjih vrednosti koncentracije IgG u krvnom serumu novorođenih teladi je zabeležen između grupe G2 i G4, gde su statistički značajno više srednje vrednosti utvrđene kod grupe G2 u odnosu na G4 u periodu 6, 24. i 48. sati po rođenju (p lt 0.01 za sve navedene vremenske periode). Relativna efikasnost apsorpcije 6-og sata po rođenju je bila statistički vrlo značajno viša (p lt 0.01) kod grupe G1 u odnosu na ostale ogledne grupe teladi.