Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods

Abstract

In the present study, isolation of essential oils from leaves of rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and thyme (Thymus vulgaris) by supercritical carbon dioxide as well as hydrodistillation was investigated from the point of yield and antimicrobial activity of obtained extracts against Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and E.coli strains. Thyme isolates had the highest antibacterial efficiency against tested foodborne bacteria strains (MIC=40-640 μg/cm3) followed by rosemary (MIC=320-1280 μg/cm3) and sage (MIC=160-2560 μg/cm3) isolates. Among tested bacteria, Bacillus species were the most sensitive to presence of all tested supercritical extracts and hydrodistillates. .

U ovoj studiji je ispitana izolacija etarskih ulja iz lista ruzmarina (Rosmarinus officinalis), žalfije (Salvia officinalis) i timijana (Thymus vulgaris) postupkom natkritične ekstrakcije ugljenik(IV)-oksidom sa aspekta prinosa i antimikrobne aktivnosti dobijenih izolata protiv Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis i E.coli sojeva. Izolati timijana su pokazali najjače antibakterijsko dejstvo u odnosu na testirane sojeve (MIC=40-640 μg/cm3). Zatim slede izolati ruzmarina (MIC=320-1280 μg/cm3) i žalfije (MIC=160-2560 μg/cm3). Među testiranim bakterijama, Bacillus sojevi su se pokazali kao najosetljiviji na prisustvo kako natkritičnih ekstrakata, tako i hidrodestilata. .