Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process

Abstract

Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160μg/mL to 1280μg/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 μg/mL to 2560 μg/mL for all examined strains except for 1 strain of S. intermedius of 320 μg/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 μg/mL.

Ispitivanjem antibakterijskog delovanja natkritičnog ekstrakta timijana (TNKE) i ekstrakta timijana dobijenog hidrodestilacijom (THD) je obuhvaćeno 18 sojeva bakterija iz rodova Staphylococcus, Streptococcus i Enterococus, uključujući sojeve MRSA, sojeve VRE kao i referentne sojeve S.pyogenes ATCC 19615, S.agalactiae ATCC 27959, S.aureus ATCC 11632 i S.aureus ATCC 25923. Antibakterijsko delovanje ekstrakata timijana ispitivano je primenom mikrodilucione metode u bujonu, a citotoksični efekat ekstrakata timijana ispitivan je na ćelijskoj liniji VERO. Dobijene vrednosti MIC THD ekstrakta kretale su se od 160 μg/mL do 1280 μg/mL za sve sojeve bakterija obuhvaćene ispitivanjem. Dobijene vrednost MIC TNKE ekstrakta iznosile su 1280 μg/mL do >2560 μg/mL za sve ispitivane sojeve osim za 1 soj S. intermedius od 320 μg/mL. Citotoksični efekat na ćelijskoj liniji VERO ispitivani ekstrakti su ispolji u koncentracijama višim od 40 μg/mL.