Приказ основних података о документу
Expression of α-SMA, desmin and vimentin in canine liver with fibrosis
Ekspresija α-SMA, dezmina i vimentina u jetri pasa sa fibrozom
dc.creator | Knežević, Milijana | |
dc.creator | Gledić, Dušan | |
dc.creator | Kukolj, Vladimir | |
dc.creator | Knežević, Đ. | |
dc.creator | Jovanović, Milan | |
dc.creator | Božić, Tatjana | |
dc.creator | Aleksić-Kovačević, Sanja | |
dc.date.accessioned | 2020-06-03T13:17:32Z | |
dc.date.available | 2020-06-03T13:17:32Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/653 | |
dc.description.abstract | In this work, 36 canine livers with fibrosis were examined. Based on the degree of fibrosis, the livers were classified into three groups: 1- mild portal fibrosis; 2- moderate portal fibrosis with mild periportal and septal fibrosis; 3- severe portal fibrosis with marked periportal and septal fibrosis or cirrhosis. Normal liver sections obtained from five dogs with no evidence of infectious, neoplastic, or cardiovascular diseases were used as controls. The degree of fibrosis was evaluated on liver sections stained according to Masson's trichrome method. It was proved using an immunohistochemical method that HSC and periductal myofibroblasts are immunoreactive to desmin, vimentin and α-SMA. Desmin, vimentin and α-SMA positive cells in the portal triad were significantly more numerous in dogs with fibrosis of the third degree than in the controls or all other examined livers. The density of α- SMA, vimentin and desmin-positive cells in the portal triad was in correlation with the degree of fibrosis, which cannot be said for HSC. These results suggest that portal myofibroblasts have a significant role in the process of liver fibrosis in dogs. | en |
dc.description.abstract | U ovom radu su prikazani rezultati ispitivanja 36 uzoraka tkiva jetre sa znacima fibroze, poreklom od pasa. Na osnovu stepena fibroze, uzorci su klasifikovani u tri grupe: 1 - blaga portalna fibroza; 2 - umerena portalna fibroza sa blagom periportalnom i septalnom fibrozom; 3 - teška portalna fibroza sa izraženom periportalnom i septalnom fibrozom ili cirozom. Kao kontrola su korišćeni uzorci tkiva jetre dobijeni od pet pasa bez znakova infekcije, neoplazije i kardiovaskularnih bolesti. Evaluacija stepena fibroze je urađena na isečcima jetre bojenim po Masson trihromnoj metodi. Imunohistohemijskom metodom je pokazano da su HSC i periduktalni miofibroblasti imunoreaktivni na dezmin, vimentin i α-SMA. Dezmin, vimentin i α-SMA, pozitivne ćelije u portalnoj trijadi bile su značajno brojnije kod pasa sa fibrozom trećeg stepena u odnosu na kontrolu i na sve druge ispitivane uzorke tkiva jetre. Gustina α-SMA, vimentin i dezmin pozitivnih ćelija u portalnoj trijadi je bila u korelaciji sa stepenom fibroze, što se ne može reći i za HSC. Ovi rezultati sugerišu da portalni miofibroblasti imaju značajnu ulogu u procesu fibroze jetre kod pasa. | sr |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/156010/RS// | |
dc.rights | openAccess | |
dc.source | Acta Veterinaria-Beograd | |
dc.subject | dogs | en |
dc.subject | HSC | en |
dc.subject | immunohistochemistry | en |
dc.subject | liver fibrosis | en |
dc.subject | myofibroblasts | en |
dc.title | Expression of α-SMA, desmin and vimentin in canine liver with fibrosis | en |
dc.title | Ekspresija α-SMA, dezmina i vimentina u jetri pasa sa fibrozom | sr |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Кнежевић, Милијана; Кнежевић, Ђ.; Јовановић, Милан; Божић, Татјана; Кукољ, Владимир; Aлексић-Ковачевић, Сања; Гледић, Душан; Експресија α-СМA, дезмина и виментина у јетри паса са фиброзом; Експресија α-СМA, дезмина и виментина у јетри паса са фиброзом; | |
dc.citation.volume | 59 | |
dc.citation.issue | 4 | |
dc.citation.spage | 361 | |
dc.citation.epage | 370 | |
dc.citation.other | 59(4): 361-370 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000270452700004 | |
dc.identifier.doi | 10.2298/AVB0904361K | |
dc.identifier.scopus | 2-s2.0-70349938041 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/1206/652.pdf | |
dc.type.version | publishedVersion |