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Primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala

dc.creatorNišavić, Jakov
dc.creatorMilić, Nenad
dc.creatorKnežević, Aleksandra
dc.creatorJovanović, Tanja
dc.date.accessioned2020-06-03T13:18:13Z
dc.date.available2020-06-03T13:18:13Z
dc.date.issued2010
dc.identifier.issn0567-8315
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/662
dc.description.abstractThe objective of this study was the application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. Twenty samples of bovine nasal swabs were separately inoculated in Vero cells. Laboratory strain TN 41 of bovine herpesvirus 1 served as a control in the experiment. The cytopathic effects were recovered in the cell line previously inoculated with a sample of bovine nasal swab, as well as in cells inoculated with the laboratory strain TN 41 of the bovine herpesvirus 1 after a period of incubation of 24h, 36h and 48h. Identification of the isolated strains of the virus was done by the virus - neutralization test with specific immune sera against BHV 1, with a titre of 1:16. Comparative analysis of DNA fragments of the laboratory strain of BHV1 and isolated strain obtained by polymerase chain reaction with primers for viral gB glycoprotein and thymidine-kinase coding region, confirmed that the isolated strain of the virus belongs to bovine herpesvirus 1 (BHV 1).en
dc.description.abstractCilj nàših istraživanja je bio primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala. Dvadeset uzoraka nosnih briseva goveda pojedinačno je inokulisano u ćelijsku liniju Vero. Referentni soj TN 41 virusa BHV 1 služio je kao pozitivna kontrola u ogledu. Posle 24h, 36h i 48h od inokulacije, utvrđena je pojava citopatogenog efekta u ćelijskoj liniji inokulisanoj uzorkom poreklom od jednog nosnog brisa goveda. Iste promene su ustanovljene i posle inokulacije referentnog soja TN 41 virusa BHV 1 u ćelijsku liniju Vero. Identifikacija izolovanog soja virusa poreklom iz uzorka nosnog brisa goveda, vršena je primenom testa virus - neutralizacije uz korišćenje specifičnog imunog seruma protiv virusa BHV 1, titra od 1:16. Uporednom analizom DNK fragmenata referentnog soja virusa BHV 1 i izolovanog soja virusa, dobijenih primenom lančane reakcije polimeraze uz korišćenje dva para prajmera koji su amplifikovali gene na molekulu DNK koji kodiraju sintezu glikoproteina B spoljašnjeg omotača virusa BHV 1 i timidin - kinaze, potvrđena je pripadnost izolovanog soja virusa goveđem herpesvirusu 1.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/20150/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145047/RS//
dc.rightsopenAccess
dc.sourceActa Veterinaria-Beograd
dc.subjectbovine herpesvirus 1 (BHV 1)en
dc.subjectVero cellsen
dc.subjectvirus -neutralization test (VN-test)en
dc.subjectvirus isolationen
dc.subjectpolymerase chain reaction(PCR)en
dc.titleThe application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samplesen
dc.titlePrimena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijalasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈовановић, Тања; Милић, Ненад; Нишавић, Јаков; Кнежевић, Aлександра; Примена ланчане реакције полимеразе у доказивању присуства говеђег херпесвируса 1 у клиничким узорцима материјала; Примена ланчане реакције полимеразе у доказивању присуства говеђег херпесвируса 1 у клиничким узорцима материјала;
dc.citation.volume60
dc.citation.issue1
dc.citation.spage39
dc.citation.epage48
dc.citation.other60(1): 39-48
dc.citation.rankM23
dc.identifier.wos000276046300005
dc.identifier.doi10.2298/AVB1001039N
dc.identifier.scopus2-s2.0-77950650435
dc.identifier.fulltexthttps://vet-erinar.vet.bg.ac.rs/bitstream/id/1215/661.pdf
dc.type.versionpublishedVersion


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