Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin
Gastroprotektivni efekat novih antidotskih kombinacija kod pacova akutno trovanih T-2 toksinom
2010
Authors
Jaćević, VesnaResanović, Radmila
Bočarov-Stančić, Aleksandra
Đorđević, Snežana
Dragojević-Simić, Viktorija
Vukajlović, Ana
Bokonjić, Dubravko
Article (Published version)
Metadata
Show full item recordAbstract
The purpose of this experiment was to evaluate the antidotal potencies of methylprednisolone (soluble form, Lemod-solu®), nimesulide, N-acetylcysteine (Fluimucil®) and their combinations in rats treated with 1.0 LD50 (0.23 mg/kg) of trichothecene mycotoxin, T-2 toxin. Their antidotal efficacy was investigated by monitoring their effects on general condition, 24-hour-survival, body weight gain, food and water consumption and pathohistological changes in the gut of Wistar rats acutely treated with a single injection of T-2 toxin during a 4-week period. The highest protective index was obtained with methylprednisolone (2.43). Initial loss of body weight (after first 7 days) was found only in T-2 toxin group. During the whole experiment, in poisoned rats protected by methylprednisolone or methylprednisolone and nimesulide, a significant increase (p lt 0.001) in body weight gain, food and water consumption in comparison with T-2 toxin group was found. At the end of the experiment, N-acetylc...ysteine, nimesulide and their combination assured higher (p lt 0.05) weight gain, food and water consumption in comparison with T-2 toxin group. Signs of hemorrhagic diathesis and necrosis of the gut crypt epithelium and lymphoid tissues were found in the T-2 toxin group. Some of these histological alterations were presented in the gut of poisoned rats treated by nimesulide, Nacetylcysteine and their combination. The gut of T-2 toxin rats treated with a combination of methylprednisolone and nimesulide and especially methylprednisolone alone had a histological structure similar to the control group. These results clearly show that methylprednisolone, a well-known anti-inflammatory and immunosuppressive drug, exerts the best antidotal effect against T-2 toxin intoxication in rats.
Cilj ovog rada je bio da se ispitaju efekti metilprednizolona (solubilni oblik, Lemod-solu®), nimesulida, N-acetilcisteina (Fluimucil®) i njihovih kombinacija kod pacova tretiranih sa 1,0 LD50 (0,23 mg/kg) trihotecenskog mikotoksina T-2. Antidotski efekat je ispitivan praćenjem njihovog uticaja na opšte stanje, 24-voro časovno preživljavanje, telesnu masu, potrošnju hrane i vode i patohistološke promene u digestivnom traktu tokom 4 nedelje, kod pacova, soj Wistar, kojima je jednokratno aplikovan T-2 toksin. Najveći zaštitni efekat ispoljio je metilprednizolon (2,43). Gubitak telesne mase, tokom prvih 7 dana ustanovljen je samo u T-2 toksin grupi. Tokom čitavog eksperimenta, u grupama trovanih pacova koji su štićeni metilprednizolonom ili metilprednizolonom i nimesulidom, ustanovljeno je značajno povećanje (p lt 0,001) telesne mase, potrošnje hrane i vode, u poređenju sa T-2 toksin gropom. Na kraju ispitivanog perioda, N-acetilcistein, nimesulid i njihova kombinacija obezbedili su takođ...e porast (p lt 0,05) telesne mase, potrošnje hrane i vode u poređenju sa T-2 toksin grupom. Znaci hemoragične dijateze i nekroza epitelnih žlezdica digestivnog trakta uočeni su u T-2 toksin grupi. Blage histološ ke promene bile su prisutne u digestivnom traktu trovanih pacova tretiranih nimesulidom, N-acetilcisteinom i njihovom kombinacijom. Histološka građa digestivnog trakta trovanih pacova koji su štićeni metilprednizolonom i nimesulidom, a posebno sa metilprednizolonom bila je slična kao u kontrolnoj grupi. Prikazani rezultati jasno ukazuju da je metilprednizolon, dobro poznat anti-inflamatorni i imunosupresivni lek, ispoljio najbolji zaštitni efekat kod pacova akutno trovanih T-2 toksinom.
Keywords:
gut / methylprednisolone / N-acetylcysteine / nimesulide / pathohistology / T-2 toxinSource:
Acta Veterinaria-Beograd, 2010, 60, 5-6, 461-478Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Projekat Ministarstva nauke Republike Srbije, br. 213017
DOI: 10.2298/AVB1006461J
ISSN: 0567-8315
WoS: 000285772100003
Scopus: 2-s2.0-78651265610
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Jaćević, Vesna AU - Resanović, Radmila AU - Bočarov-Stančić, Aleksandra AU - Đorđević, Snežana AU - Dragojević-Simić, Viktorija AU - Vukajlović, Ana AU - Bokonjić, Dubravko PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/673 AB - The purpose of this experiment was to evaluate the antidotal potencies of methylprednisolone (soluble form, Lemod-solu®), nimesulide, N-acetylcysteine (Fluimucil®) and their combinations in rats treated with 1.0 LD50 (0.23 mg/kg) of trichothecene mycotoxin, T-2 toxin. Their antidotal efficacy was investigated by monitoring their effects on general condition, 24-hour-survival, body weight gain, food and water consumption and pathohistological changes in the gut of Wistar rats acutely treated with a single injection of T-2 toxin during a 4-week period. The highest protective index was obtained with methylprednisolone (2.43). Initial loss of body weight (after first 7 days) was found only in T-2 toxin group. During the whole experiment, in poisoned rats protected by methylprednisolone or methylprednisolone and nimesulide, a significant increase (p lt 0.001) in body weight gain, food and water consumption in comparison with T-2 toxin group was found. At the end of the experiment, N-acetylcysteine, nimesulide and their combination assured higher (p lt 0.05) weight gain, food and water consumption in comparison with T-2 toxin group. Signs of hemorrhagic diathesis and necrosis of the gut crypt epithelium and lymphoid tissues were found in the T-2 toxin group. Some of these histological alterations were presented in the gut of poisoned rats treated by nimesulide, Nacetylcysteine and their combination. The gut of T-2 toxin rats treated with a combination of methylprednisolone and nimesulide and especially methylprednisolone alone had a histological structure similar to the control group. These results clearly show that methylprednisolone, a well-known anti-inflammatory and immunosuppressive drug, exerts the best antidotal effect against T-2 toxin intoxication in rats. AB - Cilj ovog rada je bio da se ispitaju efekti metilprednizolona (solubilni oblik, Lemod-solu®), nimesulida, N-acetilcisteina (Fluimucil®) i njihovih kombinacija kod pacova tretiranih sa 1,0 LD50 (0,23 mg/kg) trihotecenskog mikotoksina T-2. Antidotski efekat je ispitivan praćenjem njihovog uticaja na opšte stanje, 24-voro časovno preživljavanje, telesnu masu, potrošnju hrane i vode i patohistološke promene u digestivnom traktu tokom 4 nedelje, kod pacova, soj Wistar, kojima je jednokratno aplikovan T-2 toksin. Najveći zaštitni efekat ispoljio je metilprednizolon (2,43). Gubitak telesne mase, tokom prvih 7 dana ustanovljen je samo u T-2 toksin grupi. Tokom čitavog eksperimenta, u grupama trovanih pacova koji su štićeni metilprednizolonom ili metilprednizolonom i nimesulidom, ustanovljeno je značajno povećanje (p lt 0,001) telesne mase, potrošnje hrane i vode, u poređenju sa T-2 toksin gropom. Na kraju ispitivanog perioda, N-acetilcistein, nimesulid i njihova kombinacija obezbedili su takođe porast (p lt 0,05) telesne mase, potrošnje hrane i vode u poređenju sa T-2 toksin grupom. Znaci hemoragične dijateze i nekroza epitelnih žlezdica digestivnog trakta uočeni su u T-2 toksin grupi. Blage histološ ke promene bile su prisutne u digestivnom traktu trovanih pacova tretiranih nimesulidom, N-acetilcisteinom i njihovom kombinacijom. Histološka građa digestivnog trakta trovanih pacova koji su štićeni metilprednizolonom i nimesulidom, a posebno sa metilprednizolonom bila je slična kao u kontrolnoj grupi. Prikazani rezultati jasno ukazuju da je metilprednizolon, dobro poznat anti-inflamatorni i imunosupresivni lek, ispoljio najbolji zaštitni efekat kod pacova akutno trovanih T-2 toksinom. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin T1 - Gastroprotektivni efekat novih antidotskih kombinacija kod pacova akutno trovanih T-2 toksinom VL - 60 IS - 5-6 SP - 461 EP - 478 DO - 10.2298/AVB1006461J ER -
@article{ author = "Jaćević, Vesna and Resanović, Radmila and Bočarov-Stančić, Aleksandra and Đorđević, Snežana and Dragojević-Simić, Viktorija and Vukajlović, Ana and Bokonjić, Dubravko", year = "2010", abstract = "The purpose of this experiment was to evaluate the antidotal potencies of methylprednisolone (soluble form, Lemod-solu®), nimesulide, N-acetylcysteine (Fluimucil®) and their combinations in rats treated with 1.0 LD50 (0.23 mg/kg) of trichothecene mycotoxin, T-2 toxin. Their antidotal efficacy was investigated by monitoring their effects on general condition, 24-hour-survival, body weight gain, food and water consumption and pathohistological changes in the gut of Wistar rats acutely treated with a single injection of T-2 toxin during a 4-week period. The highest protective index was obtained with methylprednisolone (2.43). Initial loss of body weight (after first 7 days) was found only in T-2 toxin group. During the whole experiment, in poisoned rats protected by methylprednisolone or methylprednisolone and nimesulide, a significant increase (p lt 0.001) in body weight gain, food and water consumption in comparison with T-2 toxin group was found. At the end of the experiment, N-acetylcysteine, nimesulide and their combination assured higher (p lt 0.05) weight gain, food and water consumption in comparison with T-2 toxin group. Signs of hemorrhagic diathesis and necrosis of the gut crypt epithelium and lymphoid tissues were found in the T-2 toxin group. Some of these histological alterations were presented in the gut of poisoned rats treated by nimesulide, Nacetylcysteine and their combination. The gut of T-2 toxin rats treated with a combination of methylprednisolone and nimesulide and especially methylprednisolone alone had a histological structure similar to the control group. These results clearly show that methylprednisolone, a well-known anti-inflammatory and immunosuppressive drug, exerts the best antidotal effect against T-2 toxin intoxication in rats., Cilj ovog rada je bio da se ispitaju efekti metilprednizolona (solubilni oblik, Lemod-solu®), nimesulida, N-acetilcisteina (Fluimucil®) i njihovih kombinacija kod pacova tretiranih sa 1,0 LD50 (0,23 mg/kg) trihotecenskog mikotoksina T-2. Antidotski efekat je ispitivan praćenjem njihovog uticaja na opšte stanje, 24-voro časovno preživljavanje, telesnu masu, potrošnju hrane i vode i patohistološke promene u digestivnom traktu tokom 4 nedelje, kod pacova, soj Wistar, kojima je jednokratno aplikovan T-2 toksin. Najveći zaštitni efekat ispoljio je metilprednizolon (2,43). Gubitak telesne mase, tokom prvih 7 dana ustanovljen je samo u T-2 toksin grupi. Tokom čitavog eksperimenta, u grupama trovanih pacova koji su štićeni metilprednizolonom ili metilprednizolonom i nimesulidom, ustanovljeno je značajno povećanje (p lt 0,001) telesne mase, potrošnje hrane i vode, u poređenju sa T-2 toksin gropom. Na kraju ispitivanog perioda, N-acetilcistein, nimesulid i njihova kombinacija obezbedili su takođe porast (p lt 0,05) telesne mase, potrošnje hrane i vode u poređenju sa T-2 toksin grupom. Znaci hemoragične dijateze i nekroza epitelnih žlezdica digestivnog trakta uočeni su u T-2 toksin grupi. Blage histološ ke promene bile su prisutne u digestivnom traktu trovanih pacova tretiranih nimesulidom, N-acetilcisteinom i njihovom kombinacijom. Histološka građa digestivnog trakta trovanih pacova koji su štićeni metilprednizolonom i nimesulidom, a posebno sa metilprednizolonom bila je slična kao u kontrolnoj grupi. Prikazani rezultati jasno ukazuju da je metilprednizolon, dobro poznat anti-inflamatorni i imunosupresivni lek, ispoljio najbolji zaštitni efekat kod pacova akutno trovanih T-2 toksinom.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin, Gastroprotektivni efekat novih antidotskih kombinacija kod pacova akutno trovanih T-2 toksinom", volume = "60", number = "5-6", pages = "461-478", doi = "10.2298/AVB1006461J" }
Jaćević, V., Resanović, R., Bočarov-Stančić, A., Đorđević, S., Dragojević-Simić, V., Vukajlović, A.,& Bokonjić, D.. (2010). Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 461-478. https://doi.org/10.2298/AVB1006461J
Jaćević V, Resanović R, Bočarov-Stančić A, Đorđević S, Dragojević-Simić V, Vukajlović A, Bokonjić D. Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin. in Acta Veterinaria-Beograd. 2010;60(5-6):461-478. doi:10.2298/AVB1006461J .
Jaćević, Vesna, Resanović, Radmila, Bočarov-Stančić, Aleksandra, Đorđević, Snežana, Dragojević-Simić, Viktorija, Vukajlović, Ana, Bokonjić, Dubravko, "Gastroprotective effects of novel antidotal combination in rats acutely poisoned by T-2 toxin" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):461-478, https://doi.org/10.2298/AVB1006461J . .