The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro
Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro
2010
Authors
Bajić, VladanStanimirović, Zoran
Stevanović, Jevrosima
Milićević, Zorka
Živković, Lada
Spremo-Potparević, Biljana
Article (Published version)
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Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.
Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.
Keywords:
Paclitaxel (Ptx) / premature centromere division (PCD) / cycloheximide / cytokinesis block micronucleus assay(CBMN) / fluorescent in situ hybridization (FISH)Source:
Archives of Biological Sciences, 2010, 62, 1, 63-74Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
DOI: 10.2298/ABS1001063B
ISSN: 0354-4664
WoS: 000277610000009
Scopus: 2-s2.0-77955153267
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Fakultet veterinarske medicineTY - JOUR AU - Bajić, Vladan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Milićević, Zorka AU - Živković, Lada AU - Spremo-Potparević, Biljana PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/676 AB - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment. AB - Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro T1 - Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro VL - 62 IS - 1 SP - 63 EP - 74 DO - 10.2298/ABS1001063B ER -
@article{ author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Milićević, Zorka and Živković, Lada and Spremo-Potparević, Biljana", year = "2010", abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment., Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro, Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro", volume = "62", number = "1", pages = "63-74", doi = "10.2298/ABS1001063B" }
Bajić, V., Stanimirović, Z., Stevanović, J., Milićević, Z., Živković, L.,& Spremo-Potparević, B.. (2010). The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 62(1), 63-74. https://doi.org/10.2298/ABS1001063B
Bajić V, Stanimirović Z, Stevanović J, Milićević Z, Živković L, Spremo-Potparević B. The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences. 2010;62(1):63-74. doi:10.2298/ABS1001063B .
Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Milićević, Zorka, Živković, Lada, Spremo-Potparević, Biljana, "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74, https://doi.org/10.2298/ABS1001063B . .