Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows
Primena MASTIK® testa za odabir antibiotika u terapiji mastitisa krava
Abstract
In this paper comparison of efficiency of commercially available antibiotic sensitivity test MASTIK® with traditionally used Kirby-Bauer disk diffusion method was described. Six samples of examined milk were chosen on a randomly basis. In the samples presence of following microorganisms was determined: Staphylococcus aureus, Escherichia coli, coagulase-negative Staphylococcus sp. and Streptococcus uberis. Based on the result of the trial it was determined that MASTiK® test is completely comparable to the results obtained by classical disk diffusion method and can be of great help on a field in fast selection of antibiotics, while results of bacteriological identifications are being awaited.
U ovom radu izvršeno je ispitivanje u cilju poređenja primene komercijalnog MASTIK® testa za brzo određivanje osetljivosti uzročnika mastitisa krava na antibiotike i standardnog Kirby-Bauer disk-difuzionog testa. Ispitano je 6 uzoraka mleka od krava sa kliničkim i subkliničkim mastitisom, odabranih metodom slučajnog izbora. Iz uzoraka su standardnim mikrobiološkim tehnikama izolovani sledeći mikroorganizmi: Staphylococcus aureus, Escherichia coli, koagulaza-negativni Staphylococcus sp., Streptococcus dysgalactiae, Streptococcus uberis i Aspergillus fumigatus. Primenom MASTIK® testa i disk-difuzionog testa, dobijeni su u potpunosti uporedivi rezultati ispitivanja osetljivosti izolovanih bakterijskih uzročnika mastitisa na antibiotike. MASTIK® test predstavlja jednostavnu metodu, aplikativnu u terenskim uslovima i pogodnu za brz odabir antibiotika u terapiji mastitisa krava, dok se čekaju rezultati mikrobiološke identifikacije uzročnika.
Keywords:
mastitis / antibiotics / sensitivity / MASTIK® / Kirby-Bauer disc method / mastitis / antibiotici / osetljivost / MASTIK® / disk-difuzioni testSource:
Zbornik naučnih radova Instituta PKB Agroekonomik, 2010, 16, 3-4, 87-93Publisher:
- Institut PKB Agroekonomik, Padinska skela
Funding / projects:
- Biotehnološki postupci u etiološkoj dijagnostici virusnih i bakterijskih infekcija, kontaminanata hrane i bioloških proizvoda u veterinarskoj medicini (RS-MESTD-MPN2006-2010-20115)
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Petrujkić, Branko AU - Milanov, Dubravka AU - Vidić, Branka AU - Marković, Đorđe AU - Ivković, Branislav PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/680 AB - In this paper comparison of efficiency of commercially available antibiotic sensitivity test MASTIK® with traditionally used Kirby-Bauer disk diffusion method was described. Six samples of examined milk were chosen on a randomly basis. In the samples presence of following microorganisms was determined: Staphylococcus aureus, Escherichia coli, coagulase-negative Staphylococcus sp. and Streptococcus uberis. Based on the result of the trial it was determined that MASTiK® test is completely comparable to the results obtained by classical disk diffusion method and can be of great help on a field in fast selection of antibiotics, while results of bacteriological identifications are being awaited. AB - U ovom radu izvršeno je ispitivanje u cilju poređenja primene komercijalnog MASTIK® testa za brzo određivanje osetljivosti uzročnika mastitisa krava na antibiotike i standardnog Kirby-Bauer disk-difuzionog testa. Ispitano je 6 uzoraka mleka od krava sa kliničkim i subkliničkim mastitisom, odabranih metodom slučajnog izbora. Iz uzoraka su standardnim mikrobiološkim tehnikama izolovani sledeći mikroorganizmi: Staphylococcus aureus, Escherichia coli, koagulaza-negativni Staphylococcus sp., Streptococcus dysgalactiae, Streptococcus uberis i Aspergillus fumigatus. Primenom MASTIK® testa i disk-difuzionog testa, dobijeni su u potpunosti uporedivi rezultati ispitivanja osetljivosti izolovanih bakterijskih uzročnika mastitisa na antibiotike. MASTIK® test predstavlja jednostavnu metodu, aplikativnu u terenskim uslovima i pogodnu za brz odabir antibiotika u terapiji mastitisa krava, dok se čekaju rezultati mikrobiološke identifikacije uzročnika. PB - Institut PKB Agroekonomik, Padinska skela T2 - Zbornik naučnih radova Instituta PKB Agroekonomik T1 - Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows T1 - Primena MASTIK® testa za odabir antibiotika u terapiji mastitisa krava VL - 16 IS - 3-4 SP - 87 EP - 93 UR - https://hdl.handle.net/21.15107/rcub_veterinar_680 ER -
@article{ author = "Petrujkić, Branko and Milanov, Dubravka and Vidić, Branka and Marković, Đorđe and Ivković, Branislav", year = "2010", abstract = "In this paper comparison of efficiency of commercially available antibiotic sensitivity test MASTIK® with traditionally used Kirby-Bauer disk diffusion method was described. Six samples of examined milk were chosen on a randomly basis. In the samples presence of following microorganisms was determined: Staphylococcus aureus, Escherichia coli, coagulase-negative Staphylococcus sp. and Streptococcus uberis. Based on the result of the trial it was determined that MASTiK® test is completely comparable to the results obtained by classical disk diffusion method and can be of great help on a field in fast selection of antibiotics, while results of bacteriological identifications are being awaited., U ovom radu izvršeno je ispitivanje u cilju poređenja primene komercijalnog MASTIK® testa za brzo određivanje osetljivosti uzročnika mastitisa krava na antibiotike i standardnog Kirby-Bauer disk-difuzionog testa. Ispitano je 6 uzoraka mleka od krava sa kliničkim i subkliničkim mastitisom, odabranih metodom slučajnog izbora. Iz uzoraka su standardnim mikrobiološkim tehnikama izolovani sledeći mikroorganizmi: Staphylococcus aureus, Escherichia coli, koagulaza-negativni Staphylococcus sp., Streptococcus dysgalactiae, Streptococcus uberis i Aspergillus fumigatus. Primenom MASTIK® testa i disk-difuzionog testa, dobijeni su u potpunosti uporedivi rezultati ispitivanja osetljivosti izolovanih bakterijskih uzročnika mastitisa na antibiotike. MASTIK® test predstavlja jednostavnu metodu, aplikativnu u terenskim uslovima i pogodnu za brz odabir antibiotika u terapiji mastitisa krava, dok se čekaju rezultati mikrobiološke identifikacije uzročnika.", publisher = "Institut PKB Agroekonomik, Padinska skela", journal = "Zbornik naučnih radova Instituta PKB Agroekonomik", title = "Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows, Primena MASTIK® testa za odabir antibiotika u terapiji mastitisa krava", volume = "16", number = "3-4", pages = "87-93", url = "https://hdl.handle.net/21.15107/rcub_veterinar_680" }
Petrujkić, B., Milanov, D., Vidić, B., Marković, Đ.,& Ivković, B.. (2010). Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows. in Zbornik naučnih radova Instituta PKB Agroekonomik Institut PKB Agroekonomik, Padinska skela., 16(3-4), 87-93. https://hdl.handle.net/21.15107/rcub_veterinar_680
Petrujkić B, Milanov D, Vidić B, Marković Đ, Ivković B. Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2010;16(3-4):87-93. https://hdl.handle.net/21.15107/rcub_veterinar_680 .
Petrujkić, Branko, Milanov, Dubravka, Vidić, Branka, Marković, Đorđe, Ivković, Branislav, "Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows" in Zbornik naučnih radova Instituta PKB Agroekonomik, 16, no. 3-4 (2010):87-93, https://hdl.handle.net/21.15107/rcub_veterinar_680 .