Use of MASTIK® test in choice of antibiotics treatment of mastitis in cows

Abstract

In this paper comparison of efficiency of commercially available antibiotic sensitivity test MASTIK® with traditionally used Kirby-Bauer disk diffusion method was described. Six samples of examined milk were chosen on a randomly basis. In the samples presence of following microorganisms was determined: Staphylococcus aureus, Escherichia coli, coagulase-negative Staphylococcus sp. and Streptococcus uberis. Based on the result of the trial it was determined that MASTiK® test is completely comparable to the results obtained by classical disk diffusion method and can be of great help on a field in fast selection of antibiotics, while results of bacteriological identifications are being awaited.

U ovom radu izvršeno je ispitivanje u cilju poređenja primene komercijalnog MASTIK® testa za brzo određivanje osetljivosti uzročnika mastitisa krava na antibiotike i standardnog Kirby-Bauer disk-difuzionog testa. Ispitano je 6 uzoraka mleka od krava sa kliničkim i subkliničkim mastitisom, odabranih metodom slučajnog izbora. Iz uzoraka su standardnim mikrobiološkim tehnikama izolovani sledeći mikroorganizmi: Staphylococcus aureus, Escherichia coli, koagulaza-negativni Staphylococcus sp., Streptococcus dysgalactiae, Streptococcus uberis i Aspergillus fumigatus. Primenom MASTIK® testa i disk-difuzionog testa, dobijeni su u potpunosti uporedivi rezultati ispitivanja osetljivosti izolovanih bakterijskih uzročnika mastitisa na antibiotike. MASTIK® test predstavlja jednostavnu metodu, aplikativnu u terenskim uslovima i pogodnu za brz odabir antibiotika u terapiji mastitisa krava, dok se čekaju rezultati mikrobiološke identifikacije uzročnika.