Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD)
Uloga hepatičnih zvezdastih ćelija (HSCs) u razvoju fibroze jetre mačaka sa policističnim bubrezima (PKD)
2010
Аутори
Aleksić-Kovačević, SanjaKukolj, Vladimir
Kureljušić, Branislav
Marinković, Darko
Knežević, Đ.
Ignjatović, I.
Jovanović, M.
Knežević, Milijana
Gledić, Dušan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hepatic stellate cells (HSCs) play a significant role in hepatic fibrogenesis. In the following study we described the distribution of cells that express alpha-smooth muscle actin (α-SMA) and desmin in the cat liver with various degrees of fibrosis, as well as the significance of hepatic stellate cells and portal myofibroblasts in the genesis of fibrosis in cats with polycistic kidney disease. Liver samples from 15 necropsied Persian cats were examined microscopically, using H&E and Masson-trichrom methods and immunohistology for α-SMA and desmin. Liver fibrosis was confirmed in cats with terminal stage of chronic cholangiohepatitis and it was characterized by connective tissue septa which divide the liver parenchyma into irregular lobuli. Inflammation in the cat liver is connected with the activation of periductal myofibroblasts. The intensity of immunopositivity of perisinusoidal HSCs to α-SMA and desmin varied depending on the degree of fibrosis and was the strongest in livers of ca...ts with cirrhosis.
U ovom radu je opisana distribucija ćelija koje eksprimiraju α-glatkomišićni aktin (α-SMA) i dezmin u jetri mačaka sa različitim stadijumom fibroze, kao i značaj ovih ćelija u razvoju fibroze, kod mačaka sa policističnim bubrezima. Mikroskopski su ispitani uzorci jetre od 15 obdukovanih persijskih mačaka, korišćenjem HE i Mason-trihromnog bojenja i imunohistohemijski na α-SMA i dezmin. Fibroza jetre potvrđena je kod mačaka sa terminalnim stadijumom hroničnog holangiohepatitisa i odlikovala se prisustvom vezivno-tkivnih septi koje dele parenhim na nejednake lobuluse. Inflamacija u jetri mačaka u vezi je sa aktivacijom periduktalnih miofibroblasta. Intenzitet imunohistohemijske reakcije perisinusoidnih HSCs na α-SMA i dezmin razlikovala se od stepena fibroze i bio je najjači u jetri mačaka sa cirozom.
Кључне речи:
cat / liver / fibrosis / HSCs / histopathology / immunohistochemistryИзвор:
Acta Veterinaria-Beograd, 2010, 60, 4, 391-400Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
DOI: 10.2298/AVB1004391A
ISSN: 0567-8315
WoS: 000281884100007
Scopus: 2-s2.0-77957773438
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Aleksić-Kovačević, Sanja AU - Kukolj, Vladimir AU - Kureljušić, Branislav AU - Marinković, Darko AU - Knežević, Đ. AU - Ignjatović, I. AU - Jovanović, M. AU - Knežević, Milijana AU - Gledić, Dušan PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/686 AB - Hepatic stellate cells (HSCs) play a significant role in hepatic fibrogenesis. In the following study we described the distribution of cells that express alpha-smooth muscle actin (α-SMA) and desmin in the cat liver with various degrees of fibrosis, as well as the significance of hepatic stellate cells and portal myofibroblasts in the genesis of fibrosis in cats with polycistic kidney disease. Liver samples from 15 necropsied Persian cats were examined microscopically, using H&E and Masson-trichrom methods and immunohistology for α-SMA and desmin. Liver fibrosis was confirmed in cats with terminal stage of chronic cholangiohepatitis and it was characterized by connective tissue septa which divide the liver parenchyma into irregular lobuli. Inflammation in the cat liver is connected with the activation of periductal myofibroblasts. The intensity of immunopositivity of perisinusoidal HSCs to α-SMA and desmin varied depending on the degree of fibrosis and was the strongest in livers of cats with cirrhosis. AB - U ovom radu je opisana distribucija ćelija koje eksprimiraju α-glatkomišićni aktin (α-SMA) i dezmin u jetri mačaka sa različitim stadijumom fibroze, kao i značaj ovih ćelija u razvoju fibroze, kod mačaka sa policističnim bubrezima. Mikroskopski su ispitani uzorci jetre od 15 obdukovanih persijskih mačaka, korišćenjem HE i Mason-trihromnog bojenja i imunohistohemijski na α-SMA i dezmin. Fibroza jetre potvrđena je kod mačaka sa terminalnim stadijumom hroničnog holangiohepatitisa i odlikovala se prisustvom vezivno-tkivnih septi koje dele parenhim na nejednake lobuluse. Inflamacija u jetri mačaka u vezi je sa aktivacijom periduktalnih miofibroblasta. Intenzitet imunohistohemijske reakcije perisinusoidnih HSCs na α-SMA i dezmin razlikovala se od stepena fibroze i bio je najjači u jetri mačaka sa cirozom. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD) T1 - Uloga hepatičnih zvezdastih ćelija (HSCs) u razvoju fibroze jetre mačaka sa policističnim bubrezima (PKD) VL - 60 IS - 4 SP - 391 EP - 400 DO - 10.2298/AVB1004391A ER -
@article{ author = "Aleksić-Kovačević, Sanja and Kukolj, Vladimir and Kureljušić, Branislav and Marinković, Darko and Knežević, Đ. and Ignjatović, I. and Jovanović, M. and Knežević, Milijana and Gledić, Dušan", year = "2010", abstract = "Hepatic stellate cells (HSCs) play a significant role in hepatic fibrogenesis. In the following study we described the distribution of cells that express alpha-smooth muscle actin (α-SMA) and desmin in the cat liver with various degrees of fibrosis, as well as the significance of hepatic stellate cells and portal myofibroblasts in the genesis of fibrosis in cats with polycistic kidney disease. Liver samples from 15 necropsied Persian cats were examined microscopically, using H&E and Masson-trichrom methods and immunohistology for α-SMA and desmin. Liver fibrosis was confirmed in cats with terminal stage of chronic cholangiohepatitis and it was characterized by connective tissue septa which divide the liver parenchyma into irregular lobuli. Inflammation in the cat liver is connected with the activation of periductal myofibroblasts. The intensity of immunopositivity of perisinusoidal HSCs to α-SMA and desmin varied depending on the degree of fibrosis and was the strongest in livers of cats with cirrhosis., U ovom radu je opisana distribucija ćelija koje eksprimiraju α-glatkomišićni aktin (α-SMA) i dezmin u jetri mačaka sa različitim stadijumom fibroze, kao i značaj ovih ćelija u razvoju fibroze, kod mačaka sa policističnim bubrezima. Mikroskopski su ispitani uzorci jetre od 15 obdukovanih persijskih mačaka, korišćenjem HE i Mason-trihromnog bojenja i imunohistohemijski na α-SMA i dezmin. Fibroza jetre potvrđena je kod mačaka sa terminalnim stadijumom hroničnog holangiohepatitisa i odlikovala se prisustvom vezivno-tkivnih septi koje dele parenhim na nejednake lobuluse. Inflamacija u jetri mačaka u vezi je sa aktivacijom periduktalnih miofibroblasta. Intenzitet imunohistohemijske reakcije perisinusoidnih HSCs na α-SMA i dezmin razlikovala se od stepena fibroze i bio je najjači u jetri mačaka sa cirozom.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD), Uloga hepatičnih zvezdastih ćelija (HSCs) u razvoju fibroze jetre mačaka sa policističnim bubrezima (PKD)", volume = "60", number = "4", pages = "391-400", doi = "10.2298/AVB1004391A" }
Aleksić-Kovačević, S., Kukolj, V., Kureljušić, B., Marinković, D., Knežević, Đ., Ignjatović, I., Jovanović, M., Knežević, M.,& Gledić, D.. (2010). Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD). in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 391-400. https://doi.org/10.2298/AVB1004391A
Aleksić-Kovačević S, Kukolj V, Kureljušić B, Marinković D, Knežević Đ, Ignjatović I, Jovanović M, Knežević M, Gledić D. Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD). in Acta Veterinaria-Beograd. 2010;60(4):391-400. doi:10.2298/AVB1004391A .
Aleksić-Kovačević, Sanja, Kukolj, Vladimir, Kureljušić, Branislav, Marinković, Darko, Knežević, Đ., Ignjatović, I., Jovanović, M., Knežević, Milijana, Gledić, Dušan, "Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD)" in Acta Veterinaria-Beograd, 60, no. 4 (2010):391-400, https://doi.org/10.2298/AVB1004391A . .