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dc.creatorVelhner, Maja
dc.creatorMitevski, Darko
dc.creatorPotkonjak, Dubravka
dc.creatorStojanović, Dragica
dc.creatorKovačević, Mira
dc.creatorPetrović, Tamaš
dc.creatorAleksić-Kovačević, Sanja
dc.date.accessioned2020-06-03T13:21:19Z
dc.date.available2020-06-03T13:21:19Z
dc.date.issued2010
dc.identifier.issn0236-6290
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/706
dc.description.abstractThe biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log(2) at 14 days PI. The amino acid composition of VP2 in IBDVwt (222 Ala, 242 Ile, 253 Gln, 256 Ile, 279 Asp, 284 Ala, 294 Ile and 299 Ser) is described. The same sequence was found in IBDVtc, except for two point mutations, at Gln253 -> His and Ala284 -> Thr. Such amino acid substitution is responsible for partial attenuation and the ability of the strain to replicate in cell culture. None of the commercial vaccine viruses has a similar arrangement of amino acids in the variable domain of IBDV. This strongly suggests that IBDVtc originates from a very virulent strain. To the best of our knowledge, this is the first report of a concomitant infection of chickens with highly pathogenic IBDV and its mutant counterpart.en
dc.publisherAkademiai Kiado Rt, Budapest
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/20115/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/156010/RS//
dc.rightsrestrictedAccess
dc.sourceActa veterinaria Hungarica
dc.subjectIBDVen
dc.subjectCD3en
dc.subjectPCNAen
dc.subjectbursa lesion scoreen
dc.subjectantibodiesen
dc.subjectattenuationen
dc.titleBiological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flocken
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВелхнер, Маја; Ковачевић, Мира; Митевски, Дарко; Aлексић-Ковачевић, Сања; Петровић, Тамаш; Стојановић, Драгица; Поткоњак, Дубравка;
dc.citation.volume58
dc.citation.issue4
dc.citation.spage499
dc.citation.epage509
dc.citation.other58(4): 499-509
dc.citation.rankM21
dc.identifier.wos000284353900010
dc.identifier.doi10.1556/AVet.58.2010.4.10
dc.identifier.pmid21087919
dc.identifier.scopus2-s2.0-78649626950
dc.identifier.rcubconv_1742
dc.type.versionpublishedVersion


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