Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses
Abstract
In this work, Apis mellifera carnica and A. m. macedonica honey bees from Serbia, Bosnia and Herzegovina and Republic of Macedonia were analysed using molecular techniques in order to improve our knowledge about biogeography of A. mellifera on the Balkan peninsula. This is the first time that the indigenous honey bees from Bosnia and Herzegovina and Republic of Macedonia have been analyzed using a molecular approach. Sampling was carried out from 560 stationary apiaries where bees were kept in traditional hives (woven skeps). The COI-COII regions of 1680 samples were PCR-amplified and sequenced. To reveal the haplotype of studied bees, the obtained sequences were aligned with published sequence data of haplotypes that belong to A. mellifera C phylogenetic lineage. The C2D mtDNA haplotype was found in all honey bees sampled from Serbia, Bosnia and Herzegovina and Republic of Macedonia, These results show that A. m. carnica and A. m. macedonica share the same C2D mtDNA haplotype. COI gen...e segments of 1680 samples were PCR-amplified and digested with restriction enzymes NcoI and StyI in order to discriminate A. m. macedonica from A. m. carnica. Amplified fragment patterns produced by both restriction enzymes matched with diagnostic pattern characteristic for A. m. macedonica in case of samples from east, south and south-west parts of Serbia, and Republic of Macedonia, fragments of samples from northern part of Serbia and Bosnia and Herzegovina did not include NcoI, and StyI restriction sites. These results indicate that honey bees from east, south and south-west parts of Serbia, and Republic of Macedonia belong to the A. m. macedonica, and honey bees from northern part of Serbia and Bosnia and Herzegovina belong to another subspecies, probably to the A. m. carnica. Therefore A. m. macedonica has much wider area of distribution than it was previously considered.
Source:
Russian Journal of Genetics, 2010, 46, 5, 603-609Publisher:
- Maik Nauka/Interperiodica/Springer, New York
Funding / projects:
- Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance (RS-MESTD-MPN2006-2010-143022)
DOI: 10.1134/S1022795410050145
ISSN: 1022-7954
WoS: 000279086900014
Scopus: 2-s2.0-77952856518
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Jevrosima AU - Stanimirović, Zoran AU - Radaković, Milena AU - Kovačević, Sanja PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/719 AB - In this work, Apis mellifera carnica and A. m. macedonica honey bees from Serbia, Bosnia and Herzegovina and Republic of Macedonia were analysed using molecular techniques in order to improve our knowledge about biogeography of A. mellifera on the Balkan peninsula. This is the first time that the indigenous honey bees from Bosnia and Herzegovina and Republic of Macedonia have been analyzed using a molecular approach. Sampling was carried out from 560 stationary apiaries where bees were kept in traditional hives (woven skeps). The COI-COII regions of 1680 samples were PCR-amplified and sequenced. To reveal the haplotype of studied bees, the obtained sequences were aligned with published sequence data of haplotypes that belong to A. mellifera C phylogenetic lineage. The C2D mtDNA haplotype was found in all honey bees sampled from Serbia, Bosnia and Herzegovina and Republic of Macedonia, These results show that A. m. carnica and A. m. macedonica share the same C2D mtDNA haplotype. COI gene segments of 1680 samples were PCR-amplified and digested with restriction enzymes NcoI and StyI in order to discriminate A. m. macedonica from A. m. carnica. Amplified fragment patterns produced by both restriction enzymes matched with diagnostic pattern characteristic for A. m. macedonica in case of samples from east, south and south-west parts of Serbia, and Republic of Macedonia, fragments of samples from northern part of Serbia and Bosnia and Herzegovina did not include NcoI, and StyI restriction sites. These results indicate that honey bees from east, south and south-west parts of Serbia, and Republic of Macedonia belong to the A. m. macedonica, and honey bees from northern part of Serbia and Bosnia and Herzegovina belong to another subspecies, probably to the A. m. carnica. Therefore A. m. macedonica has much wider area of distribution than it was previously considered. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Genetics T1 - Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses VL - 46 IS - 5 SP - 603 EP - 609 DO - 10.1134/S1022795410050145 ER -
@article{ author = "Stevanović, Jevrosima and Stanimirović, Zoran and Radaković, Milena and Kovačević, Sanja", year = "2010", abstract = "In this work, Apis mellifera carnica and A. m. macedonica honey bees from Serbia, Bosnia and Herzegovina and Republic of Macedonia were analysed using molecular techniques in order to improve our knowledge about biogeography of A. mellifera on the Balkan peninsula. This is the first time that the indigenous honey bees from Bosnia and Herzegovina and Republic of Macedonia have been analyzed using a molecular approach. Sampling was carried out from 560 stationary apiaries where bees were kept in traditional hives (woven skeps). The COI-COII regions of 1680 samples were PCR-amplified and sequenced. To reveal the haplotype of studied bees, the obtained sequences were aligned with published sequence data of haplotypes that belong to A. mellifera C phylogenetic lineage. The C2D mtDNA haplotype was found in all honey bees sampled from Serbia, Bosnia and Herzegovina and Republic of Macedonia, These results show that A. m. carnica and A. m. macedonica share the same C2D mtDNA haplotype. COI gene segments of 1680 samples were PCR-amplified and digested with restriction enzymes NcoI and StyI in order to discriminate A. m. macedonica from A. m. carnica. Amplified fragment patterns produced by both restriction enzymes matched with diagnostic pattern characteristic for A. m. macedonica in case of samples from east, south and south-west parts of Serbia, and Republic of Macedonia, fragments of samples from northern part of Serbia and Bosnia and Herzegovina did not include NcoI, and StyI restriction sites. These results indicate that honey bees from east, south and south-west parts of Serbia, and Republic of Macedonia belong to the A. m. macedonica, and honey bees from northern part of Serbia and Bosnia and Herzegovina belong to another subspecies, probably to the A. m. carnica. Therefore A. m. macedonica has much wider area of distribution than it was previously considered.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Genetics", title = "Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses", volume = "46", number = "5", pages = "603-609", doi = "10.1134/S1022795410050145" }
Stevanović, J., Stanimirović, Z., Radaković, M.,& Kovačević, S.. (2010). Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses. in Russian Journal of Genetics Maik Nauka/Interperiodica/Springer, New York., 46(5), 603-609. https://doi.org/10.1134/S1022795410050145
Stevanović J, Stanimirović Z, Radaković M, Kovačević S. Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses. in Russian Journal of Genetics. 2010;46(5):603-609. doi:10.1134/S1022795410050145 .
Stevanović, Jevrosima, Stanimirović, Zoran, Radaković, Milena, Kovačević, Sanja, "Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses" in Russian Journal of Genetics, 46, no. 5 (2010):603-609, https://doi.org/10.1134/S1022795410050145 . .