Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis
Authorized Users Only
2010
Authors
Savić, BožidarMilićević, Vesna
Bojkovski, Jovan
Kureljušić, Branislav
Ivetić, Vojin
Pavlović, Ivan
Article (Published version)
Metadata
Show full item recordAbstract
Porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) are the most recently recognized causes of infectious hepatitis of pigs and may or may not act independently in the development of the disease. Recently it has been suggested that swine torque teno viruses (TTVs), in co-infections with some swine viral pathogens, may potentiate the severity of disease. In order to search for virological cofactors associated with infectious hepatitis in pigs, we investigated the liver tissues, to determine the presence of TTVs, PCV2 and HEV of naturally infected pigs and analysed the prevalence of both genogroups of the TTVs in the hepatitis lesions. Histopathological techniques, nested-polymerase chain reactions (nPCRs), polymerase chain reaction (PCR) and one-step reverse transcriptase polymerase chain reaction (RT-PCR) were applied to detect hepatitis lesions, TTVs genogroups 1 and 2, PCV2 and HEV infection. Of the livers examined 58% (29/50) had mild to moderate hepatitis and 74% (37/50), ...56% (28/50) and 26% (13/50) samples were nPCR, PCR and RT-PCR positive for TTVs PCV2 and HEV respectively. TTVs were detected in 84% (16/19) of the samples which were determined to be of mild severity while present in almost all (90% or 9/10) samples identified as having moderate hepatitis lesions. Additionally, the livers of 12 out of 21 (57%) pigs without the hepatitis lesions were positive for TTVs. These results demonstrate an association between TTVs and infectious hepatitis of pigs in concomitant infections with PCV2 and/or HEV and indicated that TTVs may play a role as a cofactor in the pathogenesis of disease.
Keywords:
Swine / Torque teno virus / HepatitisSource:
Veterinary Research Communications, 2010, 34, 7, 641-648Publisher:
- Springer, Dordrecht
Funding / projects:
- Razvoj i implementacija standarda dobrobiti i biosigurnosti u cilju unapređenja tehnologije proizvodnje goveda i svinja (RS-MESTD-MPN2006-2010-20110)
DOI: 10.1007/s11259-010-9432-z
ISSN: 0165-7380
PubMed: 20676762
WoS: 000281564600008
Scopus: 2-s2.0-77957595058
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Savić, Božidar AU - Milićević, Vesna AU - Bojkovski, Jovan AU - Kureljušić, Branislav AU - Ivetić, Vojin AU - Pavlović, Ivan PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/720 AB - Porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) are the most recently recognized causes of infectious hepatitis of pigs and may or may not act independently in the development of the disease. Recently it has been suggested that swine torque teno viruses (TTVs), in co-infections with some swine viral pathogens, may potentiate the severity of disease. In order to search for virological cofactors associated with infectious hepatitis in pigs, we investigated the liver tissues, to determine the presence of TTVs, PCV2 and HEV of naturally infected pigs and analysed the prevalence of both genogroups of the TTVs in the hepatitis lesions. Histopathological techniques, nested-polymerase chain reactions (nPCRs), polymerase chain reaction (PCR) and one-step reverse transcriptase polymerase chain reaction (RT-PCR) were applied to detect hepatitis lesions, TTVs genogroups 1 and 2, PCV2 and HEV infection. Of the livers examined 58% (29/50) had mild to moderate hepatitis and 74% (37/50), 56% (28/50) and 26% (13/50) samples were nPCR, PCR and RT-PCR positive for TTVs PCV2 and HEV respectively. TTVs were detected in 84% (16/19) of the samples which were determined to be of mild severity while present in almost all (90% or 9/10) samples identified as having moderate hepatitis lesions. Additionally, the livers of 12 out of 21 (57%) pigs without the hepatitis lesions were positive for TTVs. These results demonstrate an association between TTVs and infectious hepatitis of pigs in concomitant infections with PCV2 and/or HEV and indicated that TTVs may play a role as a cofactor in the pathogenesis of disease. PB - Springer, Dordrecht T2 - Veterinary Research Communications T1 - Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis VL - 34 IS - 7 SP - 641 EP - 648 DO - 10.1007/s11259-010-9432-z ER -
@article{ author = "Savić, Božidar and Milićević, Vesna and Bojkovski, Jovan and Kureljušić, Branislav and Ivetić, Vojin and Pavlović, Ivan", year = "2010", abstract = "Porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) are the most recently recognized causes of infectious hepatitis of pigs and may or may not act independently in the development of the disease. Recently it has been suggested that swine torque teno viruses (TTVs), in co-infections with some swine viral pathogens, may potentiate the severity of disease. In order to search for virological cofactors associated with infectious hepatitis in pigs, we investigated the liver tissues, to determine the presence of TTVs, PCV2 and HEV of naturally infected pigs and analysed the prevalence of both genogroups of the TTVs in the hepatitis lesions. Histopathological techniques, nested-polymerase chain reactions (nPCRs), polymerase chain reaction (PCR) and one-step reverse transcriptase polymerase chain reaction (RT-PCR) were applied to detect hepatitis lesions, TTVs genogroups 1 and 2, PCV2 and HEV infection. Of the livers examined 58% (29/50) had mild to moderate hepatitis and 74% (37/50), 56% (28/50) and 26% (13/50) samples were nPCR, PCR and RT-PCR positive for TTVs PCV2 and HEV respectively. TTVs were detected in 84% (16/19) of the samples which were determined to be of mild severity while present in almost all (90% or 9/10) samples identified as having moderate hepatitis lesions. Additionally, the livers of 12 out of 21 (57%) pigs without the hepatitis lesions were positive for TTVs. These results demonstrate an association between TTVs and infectious hepatitis of pigs in concomitant infections with PCV2 and/or HEV and indicated that TTVs may play a role as a cofactor in the pathogenesis of disease.", publisher = "Springer, Dordrecht", journal = "Veterinary Research Communications", title = "Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis", volume = "34", number = "7", pages = "641-648", doi = "10.1007/s11259-010-9432-z" }
Savić, B., Milićević, V., Bojkovski, J., Kureljušić, B., Ivetić, V.,& Pavlović, I.. (2010). Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis. in Veterinary Research Communications Springer, Dordrecht., 34(7), 641-648. https://doi.org/10.1007/s11259-010-9432-z
Savić B, Milićević V, Bojkovski J, Kureljušić B, Ivetić V, Pavlović I. Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis. in Veterinary Research Communications. 2010;34(7):641-648. doi:10.1007/s11259-010-9432-z .
Savić, Božidar, Milićević, Vesna, Bojkovski, Jovan, Kureljušić, Branislav, Ivetić, Vojin, Pavlović, Ivan, "Detection rates of the swine torque teno viruses (TTVs), porcine circovirus type 2 (PCV2) and hepatitis E virus (HEV) in the livers of pigs with hepatitis" in Veterinary Research Communications, 34, no. 7 (2010):641-648, https://doi.org/10.1007/s11259-010-9432-z . .