Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age
Siedlak, Sandra L.
Smith, Mark A.
Article (Published version)
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Chromosomal alterations are a feature of both aging and Alzheimers disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
Keywords:Age / Chromosome / Premature centromere division / Sporadic Alzheimer's disease
Source:Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2010, 65, 12, 1269-1274
- Oxford Univ Press Inc, Cary
- Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa (RS-143018)
- National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [AG028679, AG031364]