Dog saliva - a source of dog allergens
Само за регистроване кориснике
2010
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof trypsin in-gel digested protein spotsafter 2D PAGE). The allergenic pot...entialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins could be detectedwith molecular weights ranging from 14 to67 kDa. MALDI-TOF analysis of IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional IgE binding protein could bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.
Извор:
Allergy: European Journal of Allergy and Clinical Immunology, 2010, 65, 92, 105-105Издавач:
- Wiley-Blackwell, Hoboken
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Polović, N. AU - Bergman, T. AU - Milčić-Matić, Natalija AU - Gronlund, H. AU - van Hage, M. PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/737 AB - Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof trypsin in-gel digested protein spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins could be detectedwith molecular weights ranging from 14 to67 kDa. MALDI-TOF analysis of IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional IgE binding protein could bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy. PB - Wiley-Blackwell, Hoboken C3 - Allergy: European Journal of Allergy and Clinical Immunology T1 - Dog saliva - a source of dog allergens VL - 65 IS - 92 SP - 105 EP - 105 UR - https://hdl.handle.net/21.15107/rcub_cherry_1464 ER -
@conference{ author = "Polović, N. and Bergman, T. and Milčić-Matić, Natalija and Gronlund, H. and van Hage, M.", year = "2010", abstract = "Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof trypsin in-gel digested protein spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins could be detectedwith molecular weights ranging from 14 to67 kDa. MALDI-TOF analysis of IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional IgE binding protein could bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.", publisher = "Wiley-Blackwell, Hoboken", journal = "Allergy: European Journal of Allergy and Clinical Immunology", title = "Dog saliva - a source of dog allergens", volume = "65", number = "92", pages = "105-105", url = "https://hdl.handle.net/21.15107/rcub_cherry_1464" }
Polović, N., Bergman, T., Milčić-Matić, N., Gronlund, H.,& van Hage, M.. (2010). Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology Wiley-Blackwell, Hoboken., 65(92), 105-105. https://hdl.handle.net/21.15107/rcub_cherry_1464
Polović N, Bergman T, Milčić-Matić N, Gronlund H, van Hage M. Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2010;65(92):105-105. https://hdl.handle.net/21.15107/rcub_cherry_1464 .
Polović, N., Bergman, T., Milčić-Matić, Natalija, Gronlund, H., van Hage, M., "Dog saliva - a source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 65, no. 92 (2010):105-105, https://hdl.handle.net/21.15107/rcub_cherry_1464 .