Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1
Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1
2010
Autori
Milić, NenadNišavić, Jakov
Ašanin, Ružica
Knežević, Aleksandra
Ašanin, Jelena
Vidanović, Dejan
Šekler, Milanko
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.
Ključne reči:
bovine herpesvirus 1 / PCR / Real-Time PCR / goveđi herpesvirus 1 / PCR / Real-time PCRIzvor:
Veterinarski Glasnik, 2010, 64, 3-4, 159-167Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Razvoj i primena lančane reakcije polimeraze (PCR) u brzoj dijagnostici respiratorne infekcije goveda izazvane goveđim herpesvirusom 1 (RS-MESTD-MPN2006-2010-20150)
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Milić, Nenad AU - Nišavić, Jakov AU - Ašanin, Ružica AU - Knežević, Aleksandra AU - Ašanin, Jelena AU - Vidanović, Dejan AU - Šekler, Milanko PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/746 AB - Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method. AB - Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1 T1 - Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1 VL - 64 IS - 3-4 SP - 159 EP - 167 DO - 10.2298/VETGL1004159M ER -
@article{ author = "Milić, Nenad and Nišavić, Jakov and Ašanin, Ružica and Knežević, Aleksandra and Ašanin, Jelena and Vidanović, Dejan and Šekler, Milanko", year = "2010", abstract = "Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method., Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1, Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1", volume = "64", number = "3-4", pages = "159-167", doi = "10.2298/VETGL1004159M" }
Milić, N., Nišavić, J., Ašanin, R., Knežević, A., Ašanin, J., Vidanović, D.,& Šekler, M.. (2010). Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3-4), 159-167. https://doi.org/10.2298/VETGL1004159M
Milić N, Nišavić J, Ašanin R, Knežević A, Ašanin J, Vidanović D, Šekler M. Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik. 2010;64(3-4):159-167. doi:10.2298/VETGL1004159M .
Milić, Nenad, Nišavić, Jakov, Ašanin, Ružica, Knežević, Aleksandra, Ašanin, Jelena, Vidanović, Dejan, Šekler, Milanko, "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1" in Veterinarski Glasnik, 64, no. 3-4 (2010):159-167, https://doi.org/10.2298/VETGL1004159M . .