Some protein, lipoprotein and lipid alterations in serum of show jumping horses during different phases of training

Abstract

The serum total protein, lipoprotein and lipid content, together with the relative contribution of their separate fractions was evaluated in six healthy show jumping horses. The blood was sampled in the pre-jumping period, after the first (100cm) and second (120cm) jump, and again in the rest period 60 minutes after the end of the event. A significant increase of total protein level was found in the post-jumping serum sampls. The relative contribution of the protein fractions separated by paper electrophoresis was changed during the exercise. An increas in the relative amount of albumins was accompanied by a decrease in the relative amount of alpha globulins in post-jumping borse sera. The total lipoprotein, beta lipoprotein and cholesterol levels increased significantly in the samples obtained 60 minute after the jumpings. Serum lipoproteins were separated into four fractions: pre-alpha, pre-beta and beta lipoproteins, by agarose gel electrophoresis. In the rest period and during the event the alpha lipoproteins were the major fraction although in the serum samples obtained 60 minutes after the jumping, their relative amount significantly decreased, while the beta lipoprotein increased, due to the increasing needs of muscle tissue for energy.