Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering
Само за регистроване кориснике
2011
Аутори
Stanić, VojislavJanacković, Đorđe
Dimitrijević, Suzana
Tanasković, Sladjana B.
Mitrić, Miodrag
Pavlović, Mirjana S.
Krstić, Aleksandra
Jovanović, Dragoljub
Raicević, Slavica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 <= x <= 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to mor...e than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright
Кључне речи:
Hydroxyapatite / Silver / Antimicrobial / Bone / Hemolysis / AFMИзвор:
Applied Surface Science, 2011, 257, 9, 4510-4518Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Ministry of Science and Environmental Protection of the Republic of Serbia
DOI: 10.1016/j.apsusc.2010.12.113
ISSN: 0169-4332
WoS: 000286459600114
Scopus: 2-s2.0-78951487082
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stanić, Vojislav AU - Janacković, Đorđe AU - Dimitrijević, Suzana AU - Tanasković, Sladjana B. AU - Mitrić, Miodrag AU - Pavlović, Mirjana S. AU - Krstić, Aleksandra AU - Jovanović, Dragoljub AU - Raicević, Slavica PY - 2011 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/793 AB - Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 <= x <= 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright PB - Elsevier, Amsterdam T2 - Applied Surface Science T1 - Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering VL - 257 IS - 9 SP - 4510 EP - 4518 DO - 10.1016/j.apsusc.2010.12.113 ER -
@article{ author = "Stanić, Vojislav and Janacković, Đorđe and Dimitrijević, Suzana and Tanasković, Sladjana B. and Mitrić, Miodrag and Pavlović, Mirjana S. and Krstić, Aleksandra and Jovanović, Dragoljub and Raicević, Slavica", year = "2011", abstract = "Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 <= x <= 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright", publisher = "Elsevier, Amsterdam", journal = "Applied Surface Science", title = "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering", volume = "257", number = "9", pages = "4510-4518", doi = "10.1016/j.apsusc.2010.12.113" }
Stanić, V., Janacković, Đ., Dimitrijević, S., Tanasković, S. B., Mitrić, M., Pavlović, M. S., Krstić, A., Jovanović, D.,& Raicević, S.. (2011). Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science Elsevier, Amsterdam., 257(9), 4510-4518. https://doi.org/10.1016/j.apsusc.2010.12.113
Stanić V, Janacković Đ, Dimitrijević S, Tanasković SB, Mitrić M, Pavlović MS, Krstić A, Jovanović D, Raicević S. Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science. 2011;257(9):4510-4518. doi:10.1016/j.apsusc.2010.12.113 .
Stanić, Vojislav, Janacković, Đorđe, Dimitrijević, Suzana, Tanasković, Sladjana B., Mitrić, Miodrag, Pavlović, Mirjana S., Krstić, Aleksandra, Jovanović, Dragoljub, Raicević, Slavica, "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering" in Applied Surface Science, 257, no. 9 (2011):4510-4518, https://doi.org/10.1016/j.apsusc.2010.12.113 . .