Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder
Samo za registrovane korisnike
2011
Autori
Stevanović, JevrosimaStanimirović, Zoran
Genersch, Elke
Kovačević, Sanja
Ljubenković, Jovan
Radaković, Milena
Aleksić, Nevenka
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Nosema species were determined in honey bees from Balkan countries. A total of 273 Nosema-positive samples were analysed. Duplex PCR and PCR-RFLP with newly designed primers, nos-16S-fw/rv, were used to differentiate between N. apis and N. ceranae. N. apis was detected in only one sample (collected in 2008 in Serbia) and N. ceranae in all the others (N = 272) including 35 older samples from Serbia collected between 2000 and 2005. No co-infection was detected. The results suggest (1) the dominance of N. ceranae infection in all Balkan countries monitored throughout the last three years; (2) the presence of N. ceranae in Serbia at least since 2000, which means that N. ceranae has not recently displaced N. apis; (3) the higher efficacy of PCR-RFLP with newly designed primers, nos-16S-fw/rv, in comparison with duplex PCR (100%: 82%, respectively). The prevalence of N. ceranae in Balkan countries was not associated with an increase in nosemosis or colony losses resembling Colony Collapse Di...sorder (CCD).
Ključne reči:
Nosema ceranae / PCR-RFLP / duplex PCR / Balkan countriesIzvor:
Apidologie, 2011, 42, 1, 49-58Izdavač:
- Springer France, Paris
Finansiranje / projekti:
- Ekofiziološka i genetička istraživanja domaćih životinja i pčela u funkciji povećanja reproduktivnih svojstava i otpornosti na bolesti (RS-MESTD-MPN2006-2010-143022)
DOI: 10.1051/apido/2010034
ISSN: 0044-8435
WoS: 000291362500006
Scopus: 2-s2.0-80051777824
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Jevrosima AU - Stanimirović, Zoran AU - Genersch, Elke AU - Kovačević, Sanja AU - Ljubenković, Jovan AU - Radaković, Milena AU - Aleksić, Nevenka PY - 2011 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/797 AB - Nosema species were determined in honey bees from Balkan countries. A total of 273 Nosema-positive samples were analysed. Duplex PCR and PCR-RFLP with newly designed primers, nos-16S-fw/rv, were used to differentiate between N. apis and N. ceranae. N. apis was detected in only one sample (collected in 2008 in Serbia) and N. ceranae in all the others (N = 272) including 35 older samples from Serbia collected between 2000 and 2005. No co-infection was detected. The results suggest (1) the dominance of N. ceranae infection in all Balkan countries monitored throughout the last three years; (2) the presence of N. ceranae in Serbia at least since 2000, which means that N. ceranae has not recently displaced N. apis; (3) the higher efficacy of PCR-RFLP with newly designed primers, nos-16S-fw/rv, in comparison with duplex PCR (100%: 82%, respectively). The prevalence of N. ceranae in Balkan countries was not associated with an increase in nosemosis or colony losses resembling Colony Collapse Disorder (CCD). PB - Springer France, Paris T2 - Apidologie T1 - Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder VL - 42 IS - 1 SP - 49 EP - 58 DO - 10.1051/apido/2010034 ER -
@article{ author = "Stevanović, Jevrosima and Stanimirović, Zoran and Genersch, Elke and Kovačević, Sanja and Ljubenković, Jovan and Radaković, Milena and Aleksić, Nevenka", year = "2011", abstract = "Nosema species were determined in honey bees from Balkan countries. A total of 273 Nosema-positive samples were analysed. Duplex PCR and PCR-RFLP with newly designed primers, nos-16S-fw/rv, were used to differentiate between N. apis and N. ceranae. N. apis was detected in only one sample (collected in 2008 in Serbia) and N. ceranae in all the others (N = 272) including 35 older samples from Serbia collected between 2000 and 2005. No co-infection was detected. The results suggest (1) the dominance of N. ceranae infection in all Balkan countries monitored throughout the last three years; (2) the presence of N. ceranae in Serbia at least since 2000, which means that N. ceranae has not recently displaced N. apis; (3) the higher efficacy of PCR-RFLP with newly designed primers, nos-16S-fw/rv, in comparison with duplex PCR (100%: 82%, respectively). The prevalence of N. ceranae in Balkan countries was not associated with an increase in nosemosis or colony losses resembling Colony Collapse Disorder (CCD).", publisher = "Springer France, Paris", journal = "Apidologie", title = "Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder", volume = "42", number = "1", pages = "49-58", doi = "10.1051/apido/2010034" }
Stevanović, J., Stanimirović, Z., Genersch, E., Kovačević, S., Ljubenković, J., Radaković, M.,& Aleksić, N.. (2011). Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. in Apidologie Springer France, Paris., 42(1), 49-58. https://doi.org/10.1051/apido/2010034
Stevanović J, Stanimirović Z, Genersch E, Kovačević S, Ljubenković J, Radaković M, Aleksić N. Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. in Apidologie. 2011;42(1):49-58. doi:10.1051/apido/2010034 .
Stevanović, Jevrosima, Stanimirović, Zoran, Genersch, Elke, Kovačević, Sanja, Ljubenković, Jovan, Radaković, Milena, Aleksić, Nevenka, "Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder" in Apidologie, 42, no. 1 (2011):49-58, https://doi.org/10.1051/apido/2010034 . .