Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus
Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence
2011
Autori
Vasković, NikolaŠekler, Milanko
Vidanović, Dejan
Polaček, Vladimir
Kukolj, Vladimir
Matović, Kazimir
Jovanović, M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in on...e swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined.
Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, pra...ćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.
Ključne reči:
brain / H5N1 / immmunohistochemistry / pancreas / pathomorphological lesions / swanIzvor:
Acta Veterinaria-Beograd, 2011, 61, 5-6, 591-598Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB1106591V
ISSN: 0567-8315
WoS: 000299029200014
Scopus: 2-s2.0-84855845385
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Vasković, Nikola AU - Šekler, Milanko AU - Vidanović, Dejan AU - Polaček, Vladimir AU - Kukolj, Vladimir AU - Matović, Kazimir AU - Jovanović, M. PY - 2011 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/803 AB - The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined. AB - Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus T1 - Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence VL - 61 IS - 5-6 SP - 591 EP - 598 DO - 10.2298/AVB1106591V ER -
@article{ author = "Vasković, Nikola and Šekler, Milanko and Vidanović, Dejan and Polaček, Vladimir and Kukolj, Vladimir and Matović, Kazimir and Jovanović, M.", year = "2011", abstract = "The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined., Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus, Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence", volume = "61", number = "5-6", pages = "591-598", doi = "10.2298/AVB1106591V" }
Vasković, N., Šekler, M., Vidanović, D., Polaček, V., Kukolj, V., Matović, K.,& Jovanović, M.. (2011). Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 591-598. https://doi.org/10.2298/AVB1106591V
Vasković N, Šekler M, Vidanović D, Polaček V, Kukolj V, Matović K, Jovanović M. Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd. 2011;61(5-6):591-598. doi:10.2298/AVB1106591V .
Vasković, Nikola, Šekler, Milanko, Vidanović, Dejan, Polaček, Vladimir, Kukolj, Vladimir, Matović, Kazimir, Jovanović, M., "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus" in Acta Veterinaria-Beograd, 61, no. 5-6 (2011):591-598, https://doi.org/10.2298/AVB1106591V . .