Light microscopic and immunohistochemical investigation of peyers patches in calves experimentally infected with rotavirus
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Pathomorphological and immunohistochemical examinations were carried out on tissue sections of the jejunum and ileum from calves experimentally infected with rota virus immediately after birth. Rota virus particles were present in the gut lumen in absorptive enterocytes next to, and in the direct vicinity of dome epithelial cells, but were never seen inside these cells. Thus, dome epithelial cells did not reveal any participation in rota virus infection. In this work, we demonstrate that a human antiserum recognizing a phylogenetically conserved part of the CD3 epsilon cytoplasmatic tail detects T cells of calves in formalid-fixed paraffin-embedded tissue sections. This antiserum enables the morphological characterization of normal and pathological lymphoid tissues and lymphoid infiltrations in experimental work and in animal disease.
Keywords:rota virus antigen / calf intestine / CD3 peroxidasa-antiperoxidasa
Source:Acta veterinaria - Beograd, 1998, 48, 5-6, 331-337
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd