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Otpornost na kontrolisani termalni stres i tolerancija na zamrzavanje spermatozoida dve grupe nerastova

dc.creatorStanković, B.
dc.creatorHristov, Slavča
dc.creatorPetrujkić, Branko
dc.creatorDelić, N.
dc.creatorMaksimović, N.
dc.creatorBojkovski, Jovan
dc.date.accessioned2020-06-03T13:35:12Z
dc.date.available2020-06-03T13:35:12Z
dc.date.issued2012
dc.identifier.issn1450-9156
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/907
dc.description.abstractThe aim of this study was to find out is it possible to presume success of boars sperm cryopreservation, based on controlled exposure to heat stress and to investigate in vitro quality parameters of frozen/thawed sperm of previously selected 6 boars with good results (group 1) and 6 boars (group 2) with bad results on thermo resistance test (TT). In this investigation, ejaculates of 12 chosen of 75 AI boars (Landrace, Large White, Pietrain and Durock breeds) from SVC Velika Plana (Serbia) were used. Tolerance to heat stress was performed by Schaetz (1963) method. Ejaculates were extended with Androhep plus (1:1) and kept during 60 minutes on 41oC. In cryopreservation of boars semen Westendorf et al. (1975) method, modified by Bwanga et al. (1990). Very significant changes of progressive motility rate were observed after spermatozoa exposure to controlled thermal stress. High correlation coefficient of progressive motility rate of both groups of boars, but higher in group 2 for progressive motility rate after thawing and after heat stress treatment was established. Average progressive motility rate of all 12 boars did not differ before and after thawing. Presence of boars of all breeds represented in the SVC (Large White, Landrace, Duroc and Pietrain) in both quality groups confirms the assumption that suitability for cryopreservation of sperm is individual trait. According data analysis, sperm deep freezing success requires previous selection for potential donors, which have to be consider standard quality parameters testing and controlled stress exposure tests.en
dc.description.abstractCilj rada je da se ustanovi da li moguće odrediti da li je sperma nerasta pogodna za krioprezervaciju da se na osnovu izlaganja spermatozioda kontrolisanom toplotnom stresu i da se ispitaju in vitro parametri kvaliteta odmrznutog semena dve grupe od po 6 nerastova čije je seme dobro (1. grupa) ili loše podnelo toplotni stres (2. grupa). U istraživanju su korišćeni ejakulati 12 od 75 nerastova rasa: landras, veliki jorkšir, pijetren i durok (L, J, P, D) iz SVC Velika Plana, odabranih na osnovu testa termorezistencije (TT) i svrstanih u dve kvalitetne grupe. Ispitivanje stepena termorezistencije ejakulata u razređenju Androhep-om plus 1:1 60 minuta na 41 oC, je izvedeno metodom po Schaetz-u (1963). Duboko zamrzavanje sperme odabranih nerastova izvedeno modifikovanim postupkom po Westendorf-u i sar. (1975) i Bwanga-i i sar. (1990). Rezultati TT ukazali su na vrlo značajne promene progresivne pokretljivosti spermatozoida posle izlaganja kontrolisanom toplotnom stresu. Ustanovljen je visok koeficijent korelacije kod obe grupe ali ipak nešto viši kod nerastova 2. grupe za odnos procenta progresivne pokretljivosti spermatozoida posle odmrzavanja i progresivne pokretljivosti posle izlaganja temperaturi od 41oC. Prosečna progresivna pokretljivost spermatozoida svih ispitivanih nerastova se nije značajno razlikovala pre i posle zamrzavanja. Slučajni raspored nerastova svih rasa zastupljenih u SVC (Jorkšir, Landras, Durok i Pijetren) u obe kvalitetne grupe potvrđuje pretpostavku da je pogodnost sperme za krioprezervaciju individualna osobina. Za uspešnu krioprezervaciju sperme nerastova je neophodna prethodna selekcija potencijalnih donora na osnovu rezultata ispitivanja standardnih parametara kvaliteta i testova u kojima se spermatozoidi in vitro izlažu različitim kontrolisanom stresu.sr
dc.publisherInstitut za stočarstvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/20110/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31053/RS//
dc.rightsopenAccess
dc.sourceBiotechnology in Animal Husbandry
dc.subjectboaren
dc.subjectcryopreservationen
dc.subjectprogressive motility rateen
dc.subjectspermen
dc.subjectthermo resistance testen
dc.titleResistance to controlled thermal stress and tolerance to sperm cryopreservation of two groups of boarsen
dc.titleOtpornost na kontrolisani termalni stres i tolerancija na zamrzavanje spermatozoida dve grupe nerastovasr
dc.typearticle
dc.rights.licenseBY
dcterms.abstractМаксимовић, Н.; Христов, Славча; Делић, Н.; Бојковски, Јован; Станковић, Б.; Петрујкић, Бранко; Отпорност на контролисани термални стрес и толеранција на замрзавање сперматозоида две групе нерастова; Отпорност на контролисани термални стрес и толеранција на замрзавање сперматозоида две групе нерастова;
dc.citation.volume28
dc.citation.issue1
dc.citation.spage59
dc.citation.epage66
dc.citation.other28(1): 59-66
dc.citation.rankM24
dc.identifier.doi10.2298/BAH1201059S
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/1429/906.pdf
dc.identifier.rcubconv_897
dc.type.versionpublishedVersion


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