Effects of induced maternal hypothyroidism on the ovarian development of offspring rats
Uticaj indukovanog hipotireoidizma majki na razvoj jajnika mladunaca pacova
Апстракт
The effects of propylthyouracil (PTU) induced hypothyroidism of rats during pregnancy and lactation on offspring ovarian development and maturation were studied. Thyroid hormones and thyroid stimulating hormone (TSH) concentrations were determined using the radioimmunoassay method in order to verify the hypothyroid status of treated mothers and their two months old pups. The ovaries of the offspring were processed for light microscopy analysis on the day of the first estrus after the 60th day of age. Histological analysis including follicle count was performed on serial sections stained with haematoxyline/eosin and on semithin sections stained with methylene blue. A significant increase of serum TSH and decrease in T3 and T4 levels was observed in treated mothers compared to controls. The levels of measured hormones in the control and PTU-treated two months old rats were not significantly different. Ten percent of 60-dayold treated females did not reach estrus and they were sacrificed ...in diestrus. The secondary interstitial cells were the dominant structures in the ovaries. The number of healthy growing and early antral follicles was markedly decreased. Ovaries of treated rats contained relatively few antral follicles, significantly more atretic antral follicles and a decreased number of corpora lutea, compared to controls. These results indicate that lack of thyroid hormones during prenatal and early postnatal development impair ovarian development in rats.
U okviru ovog rada izučavan je uticaj hipotireoidizma majki izazvanog propiltiouracilom (PTU) tokom graviditeta i laktacije na razvoj i sazrevanje jajnika pacova. U cilju određivanja tireoidnog statusa majki i mladunaca, merena je koncentracija tireoidnih hormona i tireostimulirajućeg hormona (TSH) u serumu. Jajnici potomaka hipotireoidnih majki i kontrolne grupe sprovedeni su za analizu svetlosnom mikroskopijom na dan prvog estrusa posle 60. dana starosti. Analizirani su serijski isečci jajnika bojeni hematoksilinom i eozinom, kao i polutanki preseci bojeni metilenskim plavim. Koncentracija T4 i T3 značajno je smanjena u serumu tretiranih majki, dok je nivo TSH značajno povišen u odnosu na kontrole. Kod dva meseca starih kontrolnih i tretiranih ženskih potomaka nije bilo značajne razlike u nivou svih određivanih hormona. Deset procenata tretiranih životinja nije normalno ušlo u estrus do 60. dana, i žrtvovane su u fazi diestrusa. Sekundarne intersticijalne ćelije su dominantne struktu...re u jajnicima tretiranih pacova. Ukupan broj aktiviranih folikula je značajno smanjen uz izrazitu atreziju folikula, a smanjen je i broj žutih tela. Rezultati pokazuju da hipotireoidizam izazvan u toku graviditeta i laktacije dovodi do promena u razviću jajnika.
Кључне речи:
hypothyroidism / ovarian development / ratИзвор:
Acta Veterinaria-Beograd, 2012, 62, 5-6, 483-493Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.2298/AVB1206483R
ISSN: 0567-8315
WoS: 000313151700001
Scopus: 2-s2.0-84873911808
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Radovanović, Anita AU - Roksandić, Dragutin AU - Šimić, Marija AU - Marković, Danica AU - Gledić, Dušan PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/941 AB - The effects of propylthyouracil (PTU) induced hypothyroidism of rats during pregnancy and lactation on offspring ovarian development and maturation were studied. Thyroid hormones and thyroid stimulating hormone (TSH) concentrations were determined using the radioimmunoassay method in order to verify the hypothyroid status of treated mothers and their two months old pups. The ovaries of the offspring were processed for light microscopy analysis on the day of the first estrus after the 60th day of age. Histological analysis including follicle count was performed on serial sections stained with haematoxyline/eosin and on semithin sections stained with methylene blue. A significant increase of serum TSH and decrease in T3 and T4 levels was observed in treated mothers compared to controls. The levels of measured hormones in the control and PTU-treated two months old rats were not significantly different. Ten percent of 60-dayold treated females did not reach estrus and they were sacrificed in diestrus. The secondary interstitial cells were the dominant structures in the ovaries. The number of healthy growing and early antral follicles was markedly decreased. Ovaries of treated rats contained relatively few antral follicles, significantly more atretic antral follicles and a decreased number of corpora lutea, compared to controls. These results indicate that lack of thyroid hormones during prenatal and early postnatal development impair ovarian development in rats. AB - U okviru ovog rada izučavan je uticaj hipotireoidizma majki izazvanog propiltiouracilom (PTU) tokom graviditeta i laktacije na razvoj i sazrevanje jajnika pacova. U cilju određivanja tireoidnog statusa majki i mladunaca, merena je koncentracija tireoidnih hormona i tireostimulirajućeg hormona (TSH) u serumu. Jajnici potomaka hipotireoidnih majki i kontrolne grupe sprovedeni su za analizu svetlosnom mikroskopijom na dan prvog estrusa posle 60. dana starosti. Analizirani su serijski isečci jajnika bojeni hematoksilinom i eozinom, kao i polutanki preseci bojeni metilenskim plavim. Koncentracija T4 i T3 značajno je smanjena u serumu tretiranih majki, dok je nivo TSH značajno povišen u odnosu na kontrole. Kod dva meseca starih kontrolnih i tretiranih ženskih potomaka nije bilo značajne razlike u nivou svih određivanih hormona. Deset procenata tretiranih životinja nije normalno ušlo u estrus do 60. dana, i žrtvovane su u fazi diestrusa. Sekundarne intersticijalne ćelije su dominantne strukture u jajnicima tretiranih pacova. Ukupan broj aktiviranih folikula je značajno smanjen uz izrazitu atreziju folikula, a smanjen je i broj žutih tela. Rezultati pokazuju da hipotireoidizam izazvan u toku graviditeta i laktacije dovodi do promena u razviću jajnika. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Effects of induced maternal hypothyroidism on the ovarian development of offspring rats T1 - Uticaj indukovanog hipotireoidizma majki na razvoj jajnika mladunaca pacova VL - 62 IS - 5-6 SP - 483 EP - 493 DO - 10.2298/AVB1206483R ER -
@article{ author = "Radovanović, Anita and Roksandić, Dragutin and Šimić, Marija and Marković, Danica and Gledić, Dušan", year = "2012", abstract = "The effects of propylthyouracil (PTU) induced hypothyroidism of rats during pregnancy and lactation on offspring ovarian development and maturation were studied. Thyroid hormones and thyroid stimulating hormone (TSH) concentrations were determined using the radioimmunoassay method in order to verify the hypothyroid status of treated mothers and their two months old pups. The ovaries of the offspring were processed for light microscopy analysis on the day of the first estrus after the 60th day of age. Histological analysis including follicle count was performed on serial sections stained with haematoxyline/eosin and on semithin sections stained with methylene blue. A significant increase of serum TSH and decrease in T3 and T4 levels was observed in treated mothers compared to controls. The levels of measured hormones in the control and PTU-treated two months old rats were not significantly different. Ten percent of 60-dayold treated females did not reach estrus and they were sacrificed in diestrus. The secondary interstitial cells were the dominant structures in the ovaries. The number of healthy growing and early antral follicles was markedly decreased. Ovaries of treated rats contained relatively few antral follicles, significantly more atretic antral follicles and a decreased number of corpora lutea, compared to controls. These results indicate that lack of thyroid hormones during prenatal and early postnatal development impair ovarian development in rats., U okviru ovog rada izučavan je uticaj hipotireoidizma majki izazvanog propiltiouracilom (PTU) tokom graviditeta i laktacije na razvoj i sazrevanje jajnika pacova. U cilju određivanja tireoidnog statusa majki i mladunaca, merena je koncentracija tireoidnih hormona i tireostimulirajućeg hormona (TSH) u serumu. Jajnici potomaka hipotireoidnih majki i kontrolne grupe sprovedeni su za analizu svetlosnom mikroskopijom na dan prvog estrusa posle 60. dana starosti. Analizirani su serijski isečci jajnika bojeni hematoksilinom i eozinom, kao i polutanki preseci bojeni metilenskim plavim. Koncentracija T4 i T3 značajno je smanjena u serumu tretiranih majki, dok je nivo TSH značajno povišen u odnosu na kontrole. Kod dva meseca starih kontrolnih i tretiranih ženskih potomaka nije bilo značajne razlike u nivou svih određivanih hormona. Deset procenata tretiranih životinja nije normalno ušlo u estrus do 60. dana, i žrtvovane su u fazi diestrusa. Sekundarne intersticijalne ćelije su dominantne strukture u jajnicima tretiranih pacova. Ukupan broj aktiviranih folikula je značajno smanjen uz izrazitu atreziju folikula, a smanjen je i broj žutih tela. Rezultati pokazuju da hipotireoidizam izazvan u toku graviditeta i laktacije dovodi do promena u razviću jajnika.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Effects of induced maternal hypothyroidism on the ovarian development of offspring rats, Uticaj indukovanog hipotireoidizma majki na razvoj jajnika mladunaca pacova", volume = "62", number = "5-6", pages = "483-493", doi = "10.2298/AVB1206483R" }
Radovanović, A., Roksandić, D., Šimić, M., Marković, D.,& Gledić, D.. (2012). Effects of induced maternal hypothyroidism on the ovarian development of offspring rats. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 483-493. https://doi.org/10.2298/AVB1206483R
Radovanović A, Roksandić D, Šimić M, Marković D, Gledić D. Effects of induced maternal hypothyroidism on the ovarian development of offspring rats. in Acta Veterinaria-Beograd. 2012;62(5-6):483-493. doi:10.2298/AVB1206483R .
Radovanović, Anita, Roksandić, Dragutin, Šimić, Marija, Marković, Danica, Gledić, Dušan, "Effects of induced maternal hypothyroidism on the ovarian development of offspring rats" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):483-493, https://doi.org/10.2298/AVB1206483R . .