Prevalence of G class antibodies to antigens of lyme disease causes in dogs in Vojvodina, Serbia

Abstract

Lyme disease is a multisystemic disease, zoonotic in nature, caused by the Borrelia burgdorferi sensu lato complex. In the continent of Europe, these spirochetes are predominantly transmitted by ticks of the genus Ixodes. Small mammals and birds have particular significance as reservoirs of the cause of lyme disease. The objective of these epidemiological investigations was to determine the value of IgG seroprevalence to Borrelia burgdorferi and to secure the geographic distribution of seropositive dogs in Vojvodina. The investigations covered 135 dogs that were not vaccinated against lyme disease. The indirect ELISA test was used to determine IgG prevalence to Borrelia burgdorferi antigens. Reactive blood serums of dogs were tested again using the rapid immunochromatographic and immunoblot test. A seroprevalence of G class antibodies to antigens of lyme disease causes of 8.1% (11/135) was established in the examined dog population of Vojvodina. The biggest number of positive results was recorded for the South Bačka District. The presented value for the seroprevalence of anti-Borrelia burgdorferi antibodies in the dog population indicates the exhistence of a significant risk of humans becoming infected with the cause of lyme disease in Vojvodina.

Lajmska bolest je multisistemsko oboljenje, zoonoznog karaktera, izazvano uzročnikom Borrelia burgdorferi sensu lato complex. Ove spirohete se na evropskom kontinentu dominantno prenose krpeljima iz roda Ixodes. Poseban epidemiološki značaj imaju mali sisari i ptice kao rezervoari uzročnika lajmske bolesti. Cilj ovog epidemiološ kog istraživanja je da se utvrdi vrednost seroprevalencije IgG na Borrelia burgdorferi i sagleda geografska distribucija seropozitivnih pasa u Vojvodini. U ispitivanje je bilo uključeno 135 pasa koji nisu vakcinisani protiv lajmske bolesti. Za utvrđivanje prevalencije IgG na antigene Borrelia burgdorferi korišćen je indirektni ELISA test. Reaktivni krvni serumi pasa su ponovo testirani primenom brzog imunohromatografskog i imunoblot testa. Ustanovljena je seroprevalencija antitela klase G na antigene uzročnika lajmske bolesti od 8,1% (11/135) u populaciji pasa na području Vojvodine. Najveći broj pozitivnih rezultata je zabeležen u Južnobačkom okrugu. Navedena vrednost seroprevalencije anti-Borrelia burgdorferi antitela u populaciji pasa ukazuje na postojanje značajnog rizika za infekciju kod ljudi izazvanu uzročnikom lajmske bolesti u Vojvodini.