Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments
Samo za registrovane korisnike
2013
Autori
Petrujkić, BrankoSedej, Ivana
Beier, Ross C.
Anderson, Robin C.
Harvey, Roger B.
Epps, Sharon V. R.
Stipanović, Robert D.
Krueger, Nathan A.
Nisbet, David J.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-beta-D-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0-500 mg/L thymol. From 1 and 3 mM solutions, 0.293 +/- 0.04 and 0.898 +/- 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 +/- 0.041 and 0.317 +/- 0.143 mM thymol-beta-D-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-beta-D-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence tha...t thymol-beta-D-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut.
Ključne reči:
gas chromatography / piglet everted small intestine / thymol / thymol-beta-D-glucopyranosideIzvor:
Journal of Agricultural and Food Chemistry, 2013, 61, 15, 3757-3762Izdavač:
- Amer Chemical Soc, Washington
Finansiranje / projekti:
- Norman Borlaug Institute, USA
- USDA, ARS, SPARC, College Station, TX, USA
- Serbian Ministry of Education and Science, Belgrade, Serbia
DOI: 10.1021/jf401013a
ISSN: 0021-8561
PubMed: 23551201
WoS: 000317872700021
Scopus: 2-s2.0-84876462670
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Petrujkić, Branko AU - Sedej, Ivana AU - Beier, Ross C. AU - Anderson, Robin C. AU - Harvey, Roger B. AU - Epps, Sharon V. R. AU - Stipanović, Robert D. AU - Krueger, Nathan A. AU - Nisbet, David J. PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1016 AB - Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-beta-D-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0-500 mg/L thymol. From 1 and 3 mM solutions, 0.293 +/- 0.04 and 0.898 +/- 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 +/- 0.041 and 0.317 +/- 0.143 mM thymol-beta-D-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-beta-D-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence that thymol-beta-D-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut. PB - Amer Chemical Soc, Washington T2 - Journal of Agricultural and Food Chemistry T1 - Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments VL - 61 IS - 15 SP - 3757 EP - 3762 DO - 10.1021/jf401013a ER -
@article{ author = "Petrujkić, Branko and Sedej, Ivana and Beier, Ross C. and Anderson, Robin C. and Harvey, Roger B. and Epps, Sharon V. R. and Stipanović, Robert D. and Krueger, Nathan A. and Nisbet, David J.", year = "2013", abstract = "Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-beta-D-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0-500 mg/L thymol. From 1 and 3 mM solutions, 0.293 +/- 0.04 and 0.898 +/- 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 +/- 0.041 and 0.317 +/- 0.143 mM thymol-beta-D-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-beta-D-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence that thymol-beta-D-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Agricultural and Food Chemistry", title = "Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments", volume = "61", number = "15", pages = "3757-3762", doi = "10.1021/jf401013a" }
Petrujkić, B., Sedej, I., Beier, R. C., Anderson, R. C., Harvey, R. B., Epps, S. V. R., Stipanović, R. D., Krueger, N. A.,& Nisbet, D. J.. (2013). Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments. in Journal of Agricultural and Food Chemistry Amer Chemical Soc, Washington., 61(15), 3757-3762. https://doi.org/10.1021/jf401013a
Petrujkić B, Sedej I, Beier RC, Anderson RC, Harvey RB, Epps SVR, Stipanović RD, Krueger NA, Nisbet DJ. Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments. in Journal of Agricultural and Food Chemistry. 2013;61(15):3757-3762. doi:10.1021/jf401013a .
Petrujkić, Branko, Sedej, Ivana, Beier, Ross C., Anderson, Robin C., Harvey, Roger B., Epps, Sharon V. R., Stipanović, Robert D., Krueger, Nathan A., Nisbet, David J., "Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments" in Journal of Agricultural and Food Chemistry, 61, no. 15 (2013):3757-3762, https://doi.org/10.1021/jf401013a . .