Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
Samo za registrovane korisnike
2013
Autori
Fanovich, M. A.Ivanović, Jasna
Mišić, Dušan
Alvarez, M. V.
Jaeger, Philip
Žižović, Irena
Eggers, Rudolf
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is expose...d to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.
Ključne reči:
Polycaprolactone scaffold / Usnea extract / Supercritical extraction impregnation / Antibacterial propertiesIzvor:
Journal of Supercritical Fluids, 2013, 78, 42-53Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- National Research Council (CONICET, Argentina)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- University of Mar del Plata (Argentina)
- Technical University of Hamburg, Hamburg (Germany)
- Funkcionalni fiziološki aktivni biljni materijali sa dodatom vrednošću za primenu u farmaceutskoj i prehrambenoj industriji (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
DOI: 10.1016/j.supflu.2013.03.017
ISSN: 0896-8446
WoS: 000319854000007
Scopus: 2-s2.0-84876222754
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Fanovich, M. A. AU - Ivanović, Jasna AU - Mišić, Dušan AU - Alvarez, M. V. AU - Jaeger, Philip AU - Žižović, Irena AU - Eggers, Rudolf PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1020 AB - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Supercritical Fluids T1 - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process VL - 78 SP - 42 EP - 53 DO - 10.1016/j.supflu.2013.03.017 ER -
@article{ author = "Fanovich, M. A. and Ivanović, Jasna and Mišić, Dušan and Alvarez, M. V. and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf", year = "2013", abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Supercritical Fluids", title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process", volume = "78", pages = "42-53", doi = "10.1016/j.supflu.2013.03.017" }
Fanovich, M. A., Ivanović, J., Mišić, D., Alvarez, M. V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids Elsevier Science Bv, Amsterdam., 78, 42-53. https://doi.org/10.1016/j.supflu.2013.03.017
Fanovich MA, Ivanović J, Mišić D, Alvarez MV, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53. doi:10.1016/j.supflu.2013.03.017 .
Fanovich, M. A., Ivanović, Jasna, Mišić, Dušan, Alvarez, M. V., Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53, https://doi.org/10.1016/j.supflu.2013.03.017 . .