Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing
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2013
Authors
Vučićević, MilošStevanov-Pavlović, Marija
Stevanović, Jevrosima
Bošnjak, Jasna
Gajić, Bojan
Aleksić, Nevenka
Stanimirović, Zoran
Article (Published version)
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The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular marke...rs and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269-276, 2013.
Keywords:
molecular sexing / CHD gene / 2550F / 2718R / birdSource:
Zoo Biology, 2013, 32, 3, 269-276Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
DOI: 10.1002/zoo.21010
ISSN: 0733-3188
PubMed: 22553188
WoS: 000320139100006
Scopus: 2-s2.0-84879080670
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Fakultet veterinarske medicineTY - JOUR AU - Vučićević, Miloš AU - Stevanov-Pavlović, Marija AU - Stevanović, Jevrosima AU - Bošnjak, Jasna AU - Gajić, Bojan AU - Aleksić, Nevenka AU - Stanimirović, Zoran PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1021 AB - The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269-276, 2013. PB - Wiley-Blackwell, Hoboken T2 - Zoo Biology T1 - Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing VL - 32 IS - 3 SP - 269 EP - 276 DO - 10.1002/zoo.21010 ER -
@article{ author = "Vučićević, Miloš and Stevanov-Pavlović, Marija and Stevanović, Jevrosima and Bošnjak, Jasna and Gajić, Bojan and Aleksić, Nevenka and Stanimirović, Zoran", year = "2013", abstract = "The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269-276, 2013.", publisher = "Wiley-Blackwell, Hoboken", journal = "Zoo Biology", title = "Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing", volume = "32", number = "3", pages = "269-276", doi = "10.1002/zoo.21010" }
Vučićević, M., Stevanov-Pavlović, M., Stevanović, J., Bošnjak, J., Gajić, B., Aleksić, N.,& Stanimirović, Z.. (2013). Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. in Zoo Biology Wiley-Blackwell, Hoboken., 32(3), 269-276. https://doi.org/10.1002/zoo.21010
Vučićević M, Stevanov-Pavlović M, Stevanović J, Bošnjak J, Gajić B, Aleksić N, Stanimirović Z. Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. in Zoo Biology. 2013;32(3):269-276. doi:10.1002/zoo.21010 .
Vučićević, Miloš, Stevanov-Pavlović, Marija, Stevanović, Jevrosima, Bošnjak, Jasna, Gajić, Bojan, Aleksić, Nevenka, Stanimirović, Zoran, "Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing" in Zoo Biology, 32, no. 3 (2013):269-276, https://doi.org/10.1002/zoo.21010 . .