Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction
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2014
Authors
Nešić, KsenijaŠamanc, Horea
Vujanac, Ivan
Prodanović, Radiša
Nešić, Vladimir
Velebit, Branko
Savić, Božidar
Article (Published version)
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The ban of processed animal proteins (PAP) in feed for farmed animals led to a significant reduction of the number of BSE cases ever since bovine spongiform encephalopathy (BSE) was proven to be a feedborne disease. Although optical microscopy has been the only reference method for the detection of PAP for years, the EU legislation also foresees that other methods may be applied in addition to the microscopy, if they provide appropriate information about the origin of the animal constituents present in animal feed. This was set to become a reality in the European Union from the 1st of June 2013 when meat and bone meal (MBM) was reintroduced in fish feed. This paper presents a comparison and the possibility of combining classical microscopy and conventional polymerase chain reaction (PCR), as complementary techniques, implemented to detect the presence of fish meal and bovine, poultry, pig and mixed MBM in cattle feed. Both methods were also applied on the samples of ruminal fluid of co...ws fed feed containing MBM. The ruminal fluid was chosen to be tested in order to determine its forensic significance, as it is often not possible to perform the analysis of the incrimiated feed that has already been consumed. In contrast to the high sensitivity of microscopy, the results obtained by PCR showed substantial variability, even when applied to the isolated bones. Particles of animal origin in ruminal fluid were detected only by microscopy.
Keywords:
Bovine spongiform encephalopathy / Feed control / Processed animal proteinsSource:
Animal Feed Science and Technology, 2014, 187, 86-90Publisher:
- Elsevier, Amsterdam
Funding / projects:
DOI: 10.1016/j.anifeedsci.2013.11.001
ISSN: 0377-8401
WoS: 000329952800010
Scopus: 2-s2.0-84890803761
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Fakultet veterinarske medicineTY - JOUR AU - Nešić, Ksenija AU - Šamanc, Horea AU - Vujanac, Ivan AU - Prodanović, Radiša AU - Nešić, Vladimir AU - Velebit, Branko AU - Savić, Božidar PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1111 AB - The ban of processed animal proteins (PAP) in feed for farmed animals led to a significant reduction of the number of BSE cases ever since bovine spongiform encephalopathy (BSE) was proven to be a feedborne disease. Although optical microscopy has been the only reference method for the detection of PAP for years, the EU legislation also foresees that other methods may be applied in addition to the microscopy, if they provide appropriate information about the origin of the animal constituents present in animal feed. This was set to become a reality in the European Union from the 1st of June 2013 when meat and bone meal (MBM) was reintroduced in fish feed. This paper presents a comparison and the possibility of combining classical microscopy and conventional polymerase chain reaction (PCR), as complementary techniques, implemented to detect the presence of fish meal and bovine, poultry, pig and mixed MBM in cattle feed. Both methods were also applied on the samples of ruminal fluid of cows fed feed containing MBM. The ruminal fluid was chosen to be tested in order to determine its forensic significance, as it is often not possible to perform the analysis of the incrimiated feed that has already been consumed. In contrast to the high sensitivity of microscopy, the results obtained by PCR showed substantial variability, even when applied to the isolated bones. Particles of animal origin in ruminal fluid were detected only by microscopy. PB - Elsevier, Amsterdam T2 - Animal Feed Science and Technology T1 - Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction VL - 187 SP - 86 EP - 90 DO - 10.1016/j.anifeedsci.2013.11.001 ER -
@article{ author = "Nešić, Ksenija and Šamanc, Horea and Vujanac, Ivan and Prodanović, Radiša and Nešić, Vladimir and Velebit, Branko and Savić, Božidar", year = "2014", abstract = "The ban of processed animal proteins (PAP) in feed for farmed animals led to a significant reduction of the number of BSE cases ever since bovine spongiform encephalopathy (BSE) was proven to be a feedborne disease. Although optical microscopy has been the only reference method for the detection of PAP for years, the EU legislation also foresees that other methods may be applied in addition to the microscopy, if they provide appropriate information about the origin of the animal constituents present in animal feed. This was set to become a reality in the European Union from the 1st of June 2013 when meat and bone meal (MBM) was reintroduced in fish feed. This paper presents a comparison and the possibility of combining classical microscopy and conventional polymerase chain reaction (PCR), as complementary techniques, implemented to detect the presence of fish meal and bovine, poultry, pig and mixed MBM in cattle feed. Both methods were also applied on the samples of ruminal fluid of cows fed feed containing MBM. The ruminal fluid was chosen to be tested in order to determine its forensic significance, as it is often not possible to perform the analysis of the incrimiated feed that has already been consumed. In contrast to the high sensitivity of microscopy, the results obtained by PCR showed substantial variability, even when applied to the isolated bones. Particles of animal origin in ruminal fluid were detected only by microscopy.", publisher = "Elsevier, Amsterdam", journal = "Animal Feed Science and Technology", title = "Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction", volume = "187", pages = "86-90", doi = "10.1016/j.anifeedsci.2013.11.001" }
Nešić, K., Šamanc, H., Vujanac, I., Prodanović, R., Nešić, V., Velebit, B.,& Savić, B.. (2014). Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction. in Animal Feed Science and Technology Elsevier, Amsterdam., 187, 86-90. https://doi.org/10.1016/j.anifeedsci.2013.11.001
Nešić K, Šamanc H, Vujanac I, Prodanović R, Nešić V, Velebit B, Savić B. Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction. in Animal Feed Science and Technology. 2014;187:86-90. doi:10.1016/j.anifeedsci.2013.11.001 .
Nešić, Ksenija, Šamanc, Horea, Vujanac, Ivan, Prodanović, Radiša, Nešić, Vladimir, Velebit, Branko, Savić, Božidar, "Detection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reaction" in Animal Feed Science and Technology, 187 (2014):86-90, https://doi.org/10.1016/j.anifeedsci.2013.11.001 . .