Erythroid progenitor cells from pig bone marrow and peripheral blood
Само за регистроване кориснике
1999
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
With the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo, dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals.
Кључне речи:
erythropoietic progenitors / bone marrow / peripheral blood / pig / in vitroИзвор:
Veterinary Journal, 1999, 158, 3, 196-203Издавач:
- Elsevier Sci Ltd, Oxford
DOI: 10.1053/tvjl.1999.0382
ISSN: 1090-0233
PubMed: 10558839
WoS: 000083807600006
Scopus: 2-s2.0-0033227040
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kovačević, Milica AU - Bozić, T AU - Ivanović, Z PY - 1999 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/116 AB - With the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo, dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals. PB - Elsevier Sci Ltd, Oxford T2 - Veterinary Journal T1 - Erythroid progenitor cells from pig bone marrow and peripheral blood VL - 158 IS - 3 SP - 196 EP - 203 DO - 10.1053/tvjl.1999.0382 ER -
@article{ author = "Kovačević, Milica and Bozić, T and Ivanović, Z", year = "1999", abstract = "With the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo, dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Veterinary Journal", title = "Erythroid progenitor cells from pig bone marrow and peripheral blood", volume = "158", number = "3", pages = "196-203", doi = "10.1053/tvjl.1999.0382" }
Kovačević, M., Bozić, T.,& Ivanović, Z.. (1999). Erythroid progenitor cells from pig bone marrow and peripheral blood. in Veterinary Journal Elsevier Sci Ltd, Oxford., 158(3), 196-203. https://doi.org/10.1053/tvjl.1999.0382
Kovačević M, Bozić T, Ivanović Z. Erythroid progenitor cells from pig bone marrow and peripheral blood. in Veterinary Journal. 1999;158(3):196-203. doi:10.1053/tvjl.1999.0382 .
Kovačević, Milica, Bozić, T, Ivanović, Z, "Erythroid progenitor cells from pig bone marrow and peripheral blood" in Veterinary Journal, 158, no. 3 (1999):196-203, https://doi.org/10.1053/tvjl.1999.0382 . .