The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)
Само за регистроване кориснике
2015
Аутори
Kukolj, VladimirAleksić-Kovačević, Sanja
Katić-Radivojević, Sofija
Knežević, Dj.
Jovanović, M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The main objective of our research was to examine the role and immunophenotypic characteristics of myofibroblasts in sheep liver naturally infected by the lancet liver fluke (Dicrocoelium dendriticum). In the reported study we analyzed liver samples from 20 adult sheep, 14 infected animals and 6 controls. The liver samples were fixed in 10% buffered formalin, and routinely processed and stained using hematoxylin eosin, the periodic acid-Schiff and Masson-Goldner trichrome methods. The immunohistochemical examination was carried out by the streptavidin biotin (LSAB2) method, using antibodies for alpha-smooth muscle actin (alpha-SMA), desmin and vimentin. The histopathological examination revealed liver fibrosis in 6 out of 14 (42.9%) analyzed samples, while different forms of cholangitis were observed in the remaining 8 out of 14(57.1%). The expression of alpha-SMA was proven in perisinusoidal hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts. The degree... of alpha-SMA expression and the number of alpha-SMA immunopositive cells were the most intensive in the liver with fibrosis. Desmin expression in all liver samples of infected sheep was confirmed in hepatic stellate cells and smooth muscle cells. The hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts reacted as vimentin positive cells. In the liver without fibrotic changes hepatic stellate cells and smooth muscle cells were desmin positive. The obtained results suggest that all populations of myofibroblasts, especially hepatic stellate cells, play an important role in the increased extracellular matrix formation during parasitic liver fibrosis in sheep naturally infected with D. dendriticum.
Кључне речи:
Fibrosis / Lancet liver fluke / Liver / Myofibroblasts / SheepИзвор:
Veterinary Parasitology, 2015, 208, 3-4, 181-189Издавач:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/j.vetpar.2015.01.022
ISSN: 0304-4017
PubMed: 25683133
WoS: 000351644400010
Scopus: 2-s2.0-84923628099
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kukolj, Vladimir AU - Aleksić-Kovačević, Sanja AU - Katić-Radivojević, Sofija AU - Knežević, Dj. AU - Jovanović, M. PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1173 AB - The main objective of our research was to examine the role and immunophenotypic characteristics of myofibroblasts in sheep liver naturally infected by the lancet liver fluke (Dicrocoelium dendriticum). In the reported study we analyzed liver samples from 20 adult sheep, 14 infected animals and 6 controls. The liver samples were fixed in 10% buffered formalin, and routinely processed and stained using hematoxylin eosin, the periodic acid-Schiff and Masson-Goldner trichrome methods. The immunohistochemical examination was carried out by the streptavidin biotin (LSAB2) method, using antibodies for alpha-smooth muscle actin (alpha-SMA), desmin and vimentin. The histopathological examination revealed liver fibrosis in 6 out of 14 (42.9%) analyzed samples, while different forms of cholangitis were observed in the remaining 8 out of 14(57.1%). The expression of alpha-SMA was proven in perisinusoidal hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts. The degree of alpha-SMA expression and the number of alpha-SMA immunopositive cells were the most intensive in the liver with fibrosis. Desmin expression in all liver samples of infected sheep was confirmed in hepatic stellate cells and smooth muscle cells. The hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts reacted as vimentin positive cells. In the liver without fibrotic changes hepatic stellate cells and smooth muscle cells were desmin positive. The obtained results suggest that all populations of myofibroblasts, especially hepatic stellate cells, play an important role in the increased extracellular matrix formation during parasitic liver fibrosis in sheep naturally infected with D. dendriticum. PB - Elsevier Science Bv, Amsterdam T2 - Veterinary Parasitology T1 - The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum) VL - 208 IS - 3-4 SP - 181 EP - 189 DO - 10.1016/j.vetpar.2015.01.022 ER -
@article{ author = "Kukolj, Vladimir and Aleksić-Kovačević, Sanja and Katić-Radivojević, Sofija and Knežević, Dj. and Jovanović, M.", year = "2015", abstract = "The main objective of our research was to examine the role and immunophenotypic characteristics of myofibroblasts in sheep liver naturally infected by the lancet liver fluke (Dicrocoelium dendriticum). In the reported study we analyzed liver samples from 20 adult sheep, 14 infected animals and 6 controls. The liver samples were fixed in 10% buffered formalin, and routinely processed and stained using hematoxylin eosin, the periodic acid-Schiff and Masson-Goldner trichrome methods. The immunohistochemical examination was carried out by the streptavidin biotin (LSAB2) method, using antibodies for alpha-smooth muscle actin (alpha-SMA), desmin and vimentin. The histopathological examination revealed liver fibrosis in 6 out of 14 (42.9%) analyzed samples, while different forms of cholangitis were observed in the remaining 8 out of 14(57.1%). The expression of alpha-SMA was proven in perisinusoidal hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts. The degree of alpha-SMA expression and the number of alpha-SMA immunopositive cells were the most intensive in the liver with fibrosis. Desmin expression in all liver samples of infected sheep was confirmed in hepatic stellate cells and smooth muscle cells. The hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts reacted as vimentin positive cells. In the liver without fibrotic changes hepatic stellate cells and smooth muscle cells were desmin positive. The obtained results suggest that all populations of myofibroblasts, especially hepatic stellate cells, play an important role in the increased extracellular matrix formation during parasitic liver fibrosis in sheep naturally infected with D. dendriticum.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Veterinary Parasitology", title = "The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)", volume = "208", number = "3-4", pages = "181-189", doi = "10.1016/j.vetpar.2015.01.022" }
Kukolj, V., Aleksić-Kovačević, S., Katić-Radivojević, S., Knežević, Dj.,& Jovanović, M.. (2015). The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum). in Veterinary Parasitology Elsevier Science Bv, Amsterdam., 208(3-4), 181-189. https://doi.org/10.1016/j.vetpar.2015.01.022
Kukolj V, Aleksić-Kovačević S, Katić-Radivojević S, Knežević D, Jovanović M. The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum). in Veterinary Parasitology. 2015;208(3-4):181-189. doi:10.1016/j.vetpar.2015.01.022 .
Kukolj, Vladimir, Aleksić-Kovačević, Sanja, Katić-Radivojević, Sofija, Knežević, Dj., Jovanović, M., "The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)" in Veterinary Parasitology, 208, no. 3-4 (2015):181-189, https://doi.org/10.1016/j.vetpar.2015.01.022 . .