Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses
2015
Authors
Bulajić, SnežanaTambur, Zoran
Opacić, Dolores
Miljković-Selimović, Biljana
Doder, Radoje
Cenić-Milošević, Desanka
Article (Published version)
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Strains of Enterococcus spp. isolated from a collection of 123 artisanal and industrial cheese samples were studied for the phenotypic and genotypic assessment of antibiotic resistance. A total of 226 isolates included 119 E. faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29 E. faecalis (12.83%) and 1 E. gallinarum (0.44%). Out of 61 tested strains, 15 (24.59%) strains exhibited resistance to one or more tested antibiotics, as determined by the disc diffusion method. The resistance phenotypes were as follows: gentamicin (45.45%), tetracycline (31.82%), erythromycin (9.09%), vancomycin (9.09%) and penicillin (4.55%). The presence of tetracycline and erythromycin resistance genes [tet(M), tet(L) and erm(B), respectively] and integrase gene (int), associated with Tn916-1545 transposon family, was detected by PCR procedures. The tet(M) gene was determined in all 7 tested strains, but none of the analyzed strains harbored tet(L) determinant. The erm(B) gene was not detected i...n 9 strains characterized by phenotypic resistance to erythromycin. All 16 strains were positive for the presence of the int gene. The presented results show the presence of antibiotic resistance genes and the transposon integrase gene associated with transferable resistance in enterococci, indicating a potential for gene transfer through the food chain.
Keywords:
cheese / enterococci / antibiotic resistance / potential of antibiotic-resistant gene transferSource:
Archives of Biological Sciences, 2015, 67, 1, 139-146Publisher:
- Srpsko biološko društvo, Beograd
DOI: 10.2298/ABS140426016B
ISSN: 0354-4664
WoS: 000352003500015
Scopus: 2-s2.0-84937421809
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Fakultet veterinarske medicineTY - JOUR AU - Bulajić, Snežana AU - Tambur, Zoran AU - Opacić, Dolores AU - Miljković-Selimović, Biljana AU - Doder, Radoje AU - Cenić-Milošević, Desanka PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1196 AB - Strains of Enterococcus spp. isolated from a collection of 123 artisanal and industrial cheese samples were studied for the phenotypic and genotypic assessment of antibiotic resistance. A total of 226 isolates included 119 E. faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29 E. faecalis (12.83%) and 1 E. gallinarum (0.44%). Out of 61 tested strains, 15 (24.59%) strains exhibited resistance to one or more tested antibiotics, as determined by the disc diffusion method. The resistance phenotypes were as follows: gentamicin (45.45%), tetracycline (31.82%), erythromycin (9.09%), vancomycin (9.09%) and penicillin (4.55%). The presence of tetracycline and erythromycin resistance genes [tet(M), tet(L) and erm(B), respectively] and integrase gene (int), associated with Tn916-1545 transposon family, was detected by PCR procedures. The tet(M) gene was determined in all 7 tested strains, but none of the analyzed strains harbored tet(L) determinant. The erm(B) gene was not detected in 9 strains characterized by phenotypic resistance to erythromycin. All 16 strains were positive for the presence of the int gene. The presented results show the presence of antibiotic resistance genes and the transposon integrase gene associated with transferable resistance in enterococci, indicating a potential for gene transfer through the food chain. PB - Srpsko biološko društvo, Beograd T2 - Archives of Biological Sciences T1 - Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses VL - 67 IS - 1 SP - 139 EP - 146 DO - 10.2298/ABS140426016B ER -
@article{ author = "Bulajić, Snežana and Tambur, Zoran and Opacić, Dolores and Miljković-Selimović, Biljana and Doder, Radoje and Cenić-Milošević, Desanka", year = "2015", abstract = "Strains of Enterococcus spp. isolated from a collection of 123 artisanal and industrial cheese samples were studied for the phenotypic and genotypic assessment of antibiotic resistance. A total of 226 isolates included 119 E. faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29 E. faecalis (12.83%) and 1 E. gallinarum (0.44%). Out of 61 tested strains, 15 (24.59%) strains exhibited resistance to one or more tested antibiotics, as determined by the disc diffusion method. The resistance phenotypes were as follows: gentamicin (45.45%), tetracycline (31.82%), erythromycin (9.09%), vancomycin (9.09%) and penicillin (4.55%). The presence of tetracycline and erythromycin resistance genes [tet(M), tet(L) and erm(B), respectively] and integrase gene (int), associated with Tn916-1545 transposon family, was detected by PCR procedures. The tet(M) gene was determined in all 7 tested strains, but none of the analyzed strains harbored tet(L) determinant. The erm(B) gene was not detected in 9 strains characterized by phenotypic resistance to erythromycin. All 16 strains were positive for the presence of the int gene. The presented results show the presence of antibiotic resistance genes and the transposon integrase gene associated with transferable resistance in enterococci, indicating a potential for gene transfer through the food chain.", publisher = "Srpsko biološko društvo, Beograd", journal = "Archives of Biological Sciences", title = "Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses", volume = "67", number = "1", pages = "139-146", doi = "10.2298/ABS140426016B" }
Bulajić, S., Tambur, Z., Opacić, D., Miljković-Selimović, B., Doder, R.,& Cenić-Milošević, D.. (2015). Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses. in Archives of Biological Sciences Srpsko biološko društvo, Beograd., 67(1), 139-146. https://doi.org/10.2298/ABS140426016B
Bulajić S, Tambur Z, Opacić D, Miljković-Selimović B, Doder R, Cenić-Milošević D. Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses. in Archives of Biological Sciences. 2015;67(1):139-146. doi:10.2298/ABS140426016B .
Bulajić, Snežana, Tambur, Zoran, Opacić, Dolores, Miljković-Selimović, Biljana, Doder, Radoje, Cenić-Milošević, Desanka, "Characterization of antibiotic resistance phenotypes and resistance genes in enterococcus spp. Isolated from cheeses" in Archives of Biological Sciences, 67, no. 1 (2015):139-146, https://doi.org/10.2298/ABS140426016B . .