Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo
2015
Authors
Ozvegy, JozsefMarinković, Darko
Vučićević, Miloš
Gajić, Bojan
Stevanović, Jevrosima
Krnjaić, Dejan
Aleksić-Kovačević, Sanja
Article (Published version)
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Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed h...ematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.
Keywords:
18S rDNA / Emys orbicularis / Haemogregarina stepanowi / Serbia / cytological investigationSource:
Acta Veterinaria-Beograd, 2015, 65, 4, 443-453Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
DOI: 10.1515/acve-2015-0037
ISSN: 0567-8315
WoS: 000367467000001
Scopus: 2-s2.0-84955457760
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Fakultet veterinarske medicineTY - JOUR AU - Ozvegy, Jozsef AU - Marinković, Darko AU - Vučićević, Miloš AU - Gajić, Bojan AU - Stevanović, Jevrosima AU - Krnjaić, Dejan AU - Aleksić-Kovačević, Sanja PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1309 AB - Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo VL - 65 IS - 4 SP - 443 EP - 453 DO - 10.1515/acve-2015-0037 ER -
@article{ author = "Ozvegy, Jozsef and Marinković, Darko and Vučićević, Miloš and Gajić, Bojan and Stevanović, Jevrosima and Krnjaić, Dejan and Aleksić-Kovačević, Sanja", year = "2015", abstract = "Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo", volume = "65", number = "4", pages = "443-453", doi = "10.1515/acve-2015-0037" }
Ozvegy, J., Marinković, D., Vučićević, M., Gajić, B., Stevanović, J., Krnjaić, D.,& Aleksić-Kovačević, S.. (2015). Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 443-453. https://doi.org/10.1515/acve-2015-0037
Ozvegy J, Marinković D, Vučićević M, Gajić B, Stevanović J, Krnjaić D, Aleksić-Kovačević S. Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo. in Acta Veterinaria-Beograd. 2015;65(4):443-453. doi:10.1515/acve-2015-0037 .
Ozvegy, Jozsef, Marinković, Darko, Vučićević, Miloš, Gajić, Bojan, Stevanović, Jevrosima, Krnjaić, Dejan, Aleksić-Kovačević, Sanja, "Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo" in Acta Veterinaria-Beograd, 65, no. 4 (2015):443-453, https://doi.org/10.1515/acve-2015-0037 . .