Blood serum protein status of broilers exposed to prolonged treatment with a low dose of ochratoxin A

Abstract

The 42 day long trial was performed on Hybro broilers divided into four groups. After a 14 day preexperimental period, the groups were offered feed contaminated by 0.5 ppm ochratoxin A (OA) for 0, 7, 14 or 21 days. Blood samples were taken after the period of toxin addition and the remaining birds from the control and experimental groups were normally fed with mash without added OA until the 42nd day of the trial, when blood samples were taken again. The total level of serum proteins was not changed during of prolonged treatment with OA, but a significant increase of albumin together with a decrease of the γ-globulin fraction were noted. A/G ratio suggested that globulins were the dominant protein fraction in blood serum samples obtained from all broilers included in this trail. The concentrations of α and β globulins in serum were within the physiological limits. It could be concluded that the low dietary OA level (0.5 ppm) had a possible cumulative, but not acute effect on blood serum protein status in broilers, dependent on the duration of exposure.

Ogled je izveden na Hybro brojlerima podeljenim u četiri grupe a trajao je 42 dana. Nakon četrnaestodnevnog pripremnog periodaogledne grupe su hranjene hranom kontaminiranom ohratoksinom A u količini od 5 ppm u toku 7, 14 Hi 21 dan. Uzorci krvi za ispitivanje uzimani su nakon perioda ishrane kontaminiranom hranom a preostale jedinke hranjene su hranom bez dodatog toksina do kraja ogleda. Na kraju ogleda uzeti su uzorci krvi za ispitivanje od svih grupa. Korišćena količina OA u ispitivanom vremenu ekspozicije nema uticaj na koncentraciju ukupnih proteina. Uočeno je signifikantno povećanje albumina zajedno sa smanjenjem γ-globulina. Odnos AG ukazuje da su globulini dominantna frakcija proteina u svim ispitivanim uzorcima. Koncentracija α i β globulina u krvnom serumu kretala se u okvirima fizioloških granica. Može se zaključiti da prisustvo malih količina OA u hrani (5 ppm) poseduje moguće kumulativne ali ne i akutne efekte na proteine krvnog seruma brojlera koji zavise od vremena ekspozicije štetnom dejstvu.