Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia
2016
Authors
Maletić, MilanAleksić, Nevenka
Vejnović, Branislav
Niksić, Dragan
Kulić, Milan
Dukić, Božidar
Ćirković, Dragan
Article (Published version)
Metadata
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The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype w...as not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.
Keywords:
kappa-casein / beta-lactoglobulin / polymorphism / PCR-RFLP / Holstein-Friesian / Busha cattleSource:
Mljekarstvo, 2016, 66, 3, 198-205Publisher:
- Croatian Dairy Union, Zagreb
Funding / projects:
DOI: 10.15567/mljekarstvo.2016.0304
ISSN: 0026-704X
WoS: 000379483400004
Scopus: 2-s2.0-84979084593
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Fakultet veterinarske medicineTY - JOUR AU - Maletić, Milan AU - Aleksić, Nevenka AU - Vejnović, Branislav AU - Niksić, Dragan AU - Kulić, Milan AU - Dukić, Božidar AU - Ćirković, Dragan PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1398 AB - The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows. PB - Croatian Dairy Union, Zagreb T2 - Mljekarstvo T1 - Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia VL - 66 IS - 3 SP - 198 EP - 205 DO - 10.15567/mljekarstvo.2016.0304 ER -
@article{ author = "Maletić, Milan and Aleksić, Nevenka and Vejnović, Branislav and Niksić, Dragan and Kulić, Milan and Dukić, Božidar and Ćirković, Dragan", year = "2016", abstract = "The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.", publisher = "Croatian Dairy Union, Zagreb", journal = "Mljekarstvo", title = "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia", volume = "66", number = "3", pages = "198-205", doi = "10.15567/mljekarstvo.2016.0304" }
Maletić, M., Aleksić, N., Vejnović, B., Niksić, D., Kulić, M., Dukić, B.,& Ćirković, D.. (2016). Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo Croatian Dairy Union, Zagreb., 66(3), 198-205. https://doi.org/10.15567/mljekarstvo.2016.0304
Maletić M, Aleksić N, Vejnović B, Niksić D, Kulić M, Dukić B, Ćirković D. Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo. 2016;66(3):198-205. doi:10.15567/mljekarstvo.2016.0304 .
Maletić, Milan, Aleksić, Nevenka, Vejnović, Branislav, Niksić, Dragan, Kulić, Milan, Dukić, Božidar, Ćirković, Dragan, "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia" in Mljekarstvo, 66, no. 3 (2016):198-205, https://doi.org/10.15567/mljekarstvo.2016.0304 . .