Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay
2016
Authors
Dacić, StefanĐelić, Ninoslav
Radaković, Milena
Lakić, Nada
Veselinović, Aleksandar
Ristanić, Marko
Anđelković, Marko
Article (Published version)
Metadata
Show full item recordAbstract
Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of D...NA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.
Keywords:
composite adhesives / photopolymerisation / genotoxicity / Comet asssaySource:
Genetika, 2016, 48, 2, 617-627Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)
- Dynamics of gene pool, genetic and phenotypic variability of populations, determined by the environmental changes (RS-173012)
DOI: 10.2298/GENSR1602617D
ISSN: 0534-0012
WoS: 000389658100015
Scopus: 2-s2.0-84994493576
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Dacić, Stefan AU - Đelić, Ninoslav AU - Radaković, Milena AU - Lakić, Nada AU - Veselinović, Aleksandar AU - Ristanić, Marko AU - Anđelković, Marko PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1408 AB - Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay VL - 48 IS - 2 SP - 617 EP - 627 DO - 10.2298/GENSR1602617D ER -
@article{ author = "Dacić, Stefan and Đelić, Ninoslav and Radaković, Milena and Lakić, Nada and Veselinović, Aleksandar and Ristanić, Marko and Anđelković, Marko", year = "2016", abstract = "Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay", volume = "48", number = "2", pages = "617-627", doi = "10.2298/GENSR1602617D" }
Dacić, S., Đelić, N., Radaković, M., Lakić, N., Veselinović, A., Ristanić, M.,& Anđelković, M.. (2016). Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika Društvo genetičara Srbije, Beograd., 48(2), 617-627. https://doi.org/10.2298/GENSR1602617D
Dacić S, Đelić N, Radaković M, Lakić N, Veselinović A, Ristanić M, Anđelković M. Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika. 2016;48(2):617-627. doi:10.2298/GENSR1602617D .
Dacić, Stefan, Đelić, Ninoslav, Radaković, Milena, Lakić, Nada, Veselinović, Aleksandar, Ristanić, Marko, Anđelković, Marko, "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay" in Genetika, 48, no. 2 (2016):617-627, https://doi.org/10.2298/GENSR1602617D . .