Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action
Само за регистроване кориснике
2016
Аутори
Trailović, SašaSapundžić Zurovac, Zorana
Gruborović, S.
Marjanović, Đorđe
Nedeljković-Trailović, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). H...owever, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.
Извор:
Journal of Helminthology, 2016, 90, 6, 698-705Издавач:
- Cambridge Univ Press, Cambridge
Финансирање / пројекти:
- Развој биљних лекова и биоцида на бази карвакрола, тимола и цинамалдехида за примену у ветеринарској медицини, сточарству и производњи хране без штетних резидуа (RS-MESTD-Technological Development (TD or TR)-31087)
DOI: 10.1017/S0022149X15000978
ISSN: 0022-149X
PubMed: 26610818
WoS: 000387970900009
Scopus: 2-s2.0-84948680131
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Trailović, Saša AU - Sapundžić Zurovac, Zorana AU - Gruborović, S. AU - Marjanović, Đorđe AU - Nedeljković-Trailović, Jelena PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1425 AB - The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). However, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors. PB - Cambridge Univ Press, Cambridge T2 - Journal of Helminthology T1 - Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action VL - 90 IS - 6 SP - 698 EP - 705 DO - 10.1017/S0022149X15000978 ER -
@article{ author = "Trailović, Saša and Sapundžić Zurovac, Zorana and Gruborović, S. and Marjanović, Đorđe and Nedeljković-Trailović, Jelena", year = "2016", abstract = "The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). However, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.", publisher = "Cambridge Univ Press, Cambridge", journal = "Journal of Helminthology", title = "Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action", volume = "90", number = "6", pages = "698-705", doi = "10.1017/S0022149X15000978" }
Trailović, S., Sapundžić Zurovac, Z., Gruborović, S., Marjanović, Đ.,& Nedeljković-Trailović, J.. (2016). Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action. in Journal of Helminthology Cambridge Univ Press, Cambridge., 90(6), 698-705. https://doi.org/10.1017/S0022149X15000978
Trailović S, Sapundžić Zurovac Z, Gruborović S, Marjanović Đ, Nedeljković-Trailović J. Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action. in Journal of Helminthology. 2016;90(6):698-705. doi:10.1017/S0022149X15000978 .
Trailović, Saša, Sapundžić Zurovac, Zorana, Gruborović, S., Marjanović, Đorđe, Nedeljković-Trailović, Jelena, "Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action" in Journal of Helminthology, 90, no. 6 (2016):698-705, https://doi.org/10.1017/S0022149X15000978 . .