Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance
2017
Аутори
Kureljušić, JasnaDmitrić, Marko
Vidanović, Dejan
Teodorović, Vlado
Kureljušić, Branislav
Velhner, Maja
Karabasil, Nedjeljko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. ...The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.
Кључне речи:
pigs / Salmonella / antimicrobial resistance / PFGEИзвор:
Journal of Infection in Developing Countries, 2017, 11, 8, 640-645Издавач:
- J Infection Developing Countries, Tramaniglio
Финансирање / пројекти:
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
DOI: 10.3855/jidc.9311
ISSN: 1972-2680
PubMed: 31085825
WoS: 000412935900008
Scopus: 2-s2.0-85066260177
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kureljušić, Jasna AU - Dmitrić, Marko AU - Vidanović, Dejan AU - Teodorović, Vlado AU - Kureljušić, Branislav AU - Velhner, Maja AU - Karabasil, Nedjeljko PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1465 AB - Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter. PB - J Infection Developing Countries, Tramaniglio T2 - Journal of Infection in Developing Countries T1 - Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance VL - 11 IS - 8 SP - 640 EP - 645 DO - 10.3855/jidc.9311 ER -
@article{ author = "Kureljušić, Jasna and Dmitrić, Marko and Vidanović, Dejan and Teodorović, Vlado and Kureljušić, Branislav and Velhner, Maja and Karabasil, Nedjeljko", year = "2017", abstract = "Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.", publisher = "J Infection Developing Countries, Tramaniglio", journal = "Journal of Infection in Developing Countries", title = "Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance", volume = "11", number = "8", pages = "640-645", doi = "10.3855/jidc.9311" }
Kureljušić, J., Dmitrić, M., Vidanović, D., Teodorović, V., Kureljušić, B., Velhner, M.,& Karabasil, N.. (2017). Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance. in Journal of Infection in Developing Countries J Infection Developing Countries, Tramaniglio., 11(8), 640-645. https://doi.org/10.3855/jidc.9311
Kureljušić J, Dmitrić M, Vidanović D, Teodorović V, Kureljušić B, Velhner M, Karabasil N. Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance. in Journal of Infection in Developing Countries. 2017;11(8):640-645. doi:10.3855/jidc.9311 .
Kureljušić, Jasna, Dmitrić, Marko, Vidanović, Dejan, Teodorović, Vlado, Kureljušić, Branislav, Velhner, Maja, Karabasil, Nedjeljko, "Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance" in Journal of Infection in Developing Countries, 11, no. 8 (2017):640-645, https://doi.org/10.3855/jidc.9311 . .