Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs
Apstrakt
Background: Cerebral amyloid angiopathy (CAA) is a disorder characterized by amyloid deposition in the wall of cerebral blood vessels. The deposits of amyloid occur frequently in the blood vessels of the frontal, parietal and occipital cortex. Objective: To examine the characteristics of CAA classified according to the Vonsattel scale in elderly dogs histologically and immunohistochemically as well as the semi-quantitative evaluation of the amyloid deposits in the different segments of the brain. Animals and methods: The brains of 36 dogs of different breeds and sexes, which had been routinely necropsied, were used and divided into two groups: dogs from 1 to 5 and 10 to 18 years old. The tissue sections were stained by hematoxylin-eosin, Congo red and immunohistochemically. Results: Amyloid was accumulated in the wall of cerebral blood vessels in 70% of dogs over the age of 10 years predominantly in the frontal cortex. CAA was demonstrated in elderly dogs as follows: in the frontal cor...tex (n=19 or 63%), the parietal cortex (n=12 or 40%), the hippocampus (40%) and the cerebellum (n=5 or 17%). The deposits of amyloid in the wall of blood vessels detected by Congo red staining were also A beta 1-14 and A beta 1-42 immunohistochemically positive. Most commonly, the amyloid deposits affected a moderate number of blood vessels. The accumulation of amyloid was immunohistochemically revealed in the blood vessel walls as well as in the senile plaques and neurons. Conclusion: The amount of amyloid in the arterial walls increased with age in dogs, whereas the amyloid accumulated in plaques was Congo red negative.
Ključne reči:
Dog / canine / brain / amyloid / angiopathyIzvor:
Veterinary Quarterly, 2017, 37, 1, 1-7Izdavač:
- Taylor & Francis Ltd, Abingdon
DOI: 10.1080/01652176.2016.1235301
ISSN: 0165-2176
PubMed: 27669976
WoS: 000399584500001
Scopus: 2-s2.0-85028851512
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Nešić, Slađan AU - Kukolj, Vladimir AU - Marinković, Darko AU - Vučićević, Ivana AU - Jovanović, Milijan PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1508 AB - Background: Cerebral amyloid angiopathy (CAA) is a disorder characterized by amyloid deposition in the wall of cerebral blood vessels. The deposits of amyloid occur frequently in the blood vessels of the frontal, parietal and occipital cortex. Objective: To examine the characteristics of CAA classified according to the Vonsattel scale in elderly dogs histologically and immunohistochemically as well as the semi-quantitative evaluation of the amyloid deposits in the different segments of the brain. Animals and methods: The brains of 36 dogs of different breeds and sexes, which had been routinely necropsied, were used and divided into two groups: dogs from 1 to 5 and 10 to 18 years old. The tissue sections were stained by hematoxylin-eosin, Congo red and immunohistochemically. Results: Amyloid was accumulated in the wall of cerebral blood vessels in 70% of dogs over the age of 10 years predominantly in the frontal cortex. CAA was demonstrated in elderly dogs as follows: in the frontal cortex (n=19 or 63%), the parietal cortex (n=12 or 40%), the hippocampus (40%) and the cerebellum (n=5 or 17%). The deposits of amyloid in the wall of blood vessels detected by Congo red staining were also A beta 1-14 and A beta 1-42 immunohistochemically positive. Most commonly, the amyloid deposits affected a moderate number of blood vessels. The accumulation of amyloid was immunohistochemically revealed in the blood vessel walls as well as in the senile plaques and neurons. Conclusion: The amount of amyloid in the arterial walls increased with age in dogs, whereas the amyloid accumulated in plaques was Congo red negative. PB - Taylor & Francis Ltd, Abingdon T2 - Veterinary Quarterly T1 - Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs VL - 37 IS - 1 SP - 1 EP - 7 DO - 10.1080/01652176.2016.1235301 ER -
@article{ author = "Nešić, Slađan and Kukolj, Vladimir and Marinković, Darko and Vučićević, Ivana and Jovanović, Milijan", year = "2017", abstract = "Background: Cerebral amyloid angiopathy (CAA) is a disorder characterized by amyloid deposition in the wall of cerebral blood vessels. The deposits of amyloid occur frequently in the blood vessels of the frontal, parietal and occipital cortex. Objective: To examine the characteristics of CAA classified according to the Vonsattel scale in elderly dogs histologically and immunohistochemically as well as the semi-quantitative evaluation of the amyloid deposits in the different segments of the brain. Animals and methods: The brains of 36 dogs of different breeds and sexes, which had been routinely necropsied, were used and divided into two groups: dogs from 1 to 5 and 10 to 18 years old. The tissue sections were stained by hematoxylin-eosin, Congo red and immunohistochemically. Results: Amyloid was accumulated in the wall of cerebral blood vessels in 70% of dogs over the age of 10 years predominantly in the frontal cortex. CAA was demonstrated in elderly dogs as follows: in the frontal cortex (n=19 or 63%), the parietal cortex (n=12 or 40%), the hippocampus (40%) and the cerebellum (n=5 or 17%). The deposits of amyloid in the wall of blood vessels detected by Congo red staining were also A beta 1-14 and A beta 1-42 immunohistochemically positive. Most commonly, the amyloid deposits affected a moderate number of blood vessels. The accumulation of amyloid was immunohistochemically revealed in the blood vessel walls as well as in the senile plaques and neurons. Conclusion: The amount of amyloid in the arterial walls increased with age in dogs, whereas the amyloid accumulated in plaques was Congo red negative.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Veterinary Quarterly", title = "Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs", volume = "37", number = "1", pages = "1-7", doi = "10.1080/01652176.2016.1235301" }
Nešić, S., Kukolj, V., Marinković, D., Vučićević, I.,& Jovanović, M.. (2017). Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs. in Veterinary Quarterly Taylor & Francis Ltd, Abingdon., 37(1), 1-7. https://doi.org/10.1080/01652176.2016.1235301
Nešić S, Kukolj V, Marinković D, Vučićević I, Jovanović M. Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs. in Veterinary Quarterly. 2017;37(1):1-7. doi:10.1080/01652176.2016.1235301 .
Nešić, Slađan, Kukolj, Vladimir, Marinković, Darko, Vučićević, Ivana, Jovanović, Milijan, "Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs" in Veterinary Quarterly, 37, no. 1 (2017):1-7, https://doi.org/10.1080/01652176.2016.1235301 . .