Honey bee viruses in Serbian colonies of different strength
2018
Authors
Ćirković, DraganStevanović, Jevrosima
Glavinić, Uroš
Aleksić, Nevenka
Đurić, Spomenka
Aleksić, Jelena
Stanimirović, Zoran
Article (Published version)
Metadata
Show full item recordAbstract
Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for D...WV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.
Keywords:
Apis mellifera / Viruses / Colony strength / Phylogeny / Beekeeping / Bee pathologySource:
PEERJ, 2018, 6, e5887-Publisher:
- Peerj Inc, London
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.7717/peerj.5887
ISSN: 2167-8359
PubMed: 30479890
WoS: 000452448500002
Scopus: 2-s2.0-85056855523
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Ćirković, Dragan AU - Stevanović, Jevrosima AU - Glavinić, Uroš AU - Aleksić, Nevenka AU - Đurić, Spomenka AU - Aleksić, Jelena AU - Stanimirović, Zoran PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1572 AB - Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank. PB - Peerj Inc, London T2 - PEERJ T1 - Honey bee viruses in Serbian colonies of different strength VL - 6 SP - e5887 DO - 10.7717/peerj.5887 ER -
@article{ author = "Ćirković, Dragan and Stevanović, Jevrosima and Glavinić, Uroš and Aleksić, Nevenka and Đurić, Spomenka and Aleksić, Jelena and Stanimirović, Zoran", year = "2018", abstract = "Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.", publisher = "Peerj Inc, London", journal = "PEERJ", title = "Honey bee viruses in Serbian colonies of different strength", volume = "6", pages = "e5887", doi = "10.7717/peerj.5887" }
Ćirković, D., Stevanović, J., Glavinić, U., Aleksić, N., Đurić, S., Aleksić, J.,& Stanimirović, Z.. (2018). Honey bee viruses in Serbian colonies of different strength. in PEERJ Peerj Inc, London., 6, e5887. https://doi.org/10.7717/peerj.5887
Ćirković D, Stevanović J, Glavinić U, Aleksić N, Đurić S, Aleksić J, Stanimirović Z. Honey bee viruses in Serbian colonies of different strength. in PEERJ. 2018;6:e5887. doi:10.7717/peerj.5887 .
Ćirković, Dragan, Stevanović, Jevrosima, Glavinić, Uroš, Aleksić, Nevenka, Đurić, Spomenka, Aleksić, Jelena, Stanimirović, Zoran, "Honey bee viruses in Serbian colonies of different strength" in PEERJ, 6 (2018):e5887, https://doi.org/10.7717/peerj.5887 . .