Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia
2018
Authors
Radalj, AndreaNišavić, Jakov
Krnjaić, Dejan
Valčić, Miroslav
Jovanović, Tanja
Veljović, Ljubiša
Milić, Nenad
Article (Published version)
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The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clu...stered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.
Keywords:
EHV-1 / EHV-2 / EHV-5 / virus isolation / molecular characterizationSource:
Acta Veterinaria - Brno, 2018, 87, 1, 27-Publisher:
- Veterinarni A Farmaceuticka Univerzita Brno, Brno
Funding / projects:
- Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens (RS-MESTD-Technological Development (TD or TR)-31008)
DOI: 10.2754/avb201887010027
ISSN: 0001-7213
WoS: 000431956300004
Scopus: 2-s2.0-85045414513
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Fakultet veterinarske medicineTY - JOUR AU - Radalj, Andrea AU - Nišavić, Jakov AU - Krnjaić, Dejan AU - Valčić, Miroslav AU - Jovanović, Tanja AU - Veljović, Ljubiša AU - Milić, Nenad PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1626 AB - The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains. PB - Veterinarni A Farmaceuticka Univerzita Brno, Brno T2 - Acta Veterinaria - Brno T1 - Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia VL - 87 IS - 1 SP - 27 DO - 10.2754/avb201887010027 ER -
@article{ author = "Radalj, Andrea and Nišavić, Jakov and Krnjaić, Dejan and Valčić, Miroslav and Jovanović, Tanja and Veljović, Ljubiša and Milić, Nenad", year = "2018", abstract = "The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.", publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno", journal = "Acta Veterinaria - Brno", title = "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia", volume = "87", number = "1", pages = "27", doi = "10.2754/avb201887010027" }
Radalj, A., Nišavić, J., Krnjaić, D., Valčić, M., Jovanović, T., Veljović, L.,& Milić, N.. (2018). Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(1), 27. https://doi.org/10.2754/avb201887010027
Radalj A, Nišavić J, Krnjaić D, Valčić M, Jovanović T, Veljović L, Milić N. Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno. 2018;87(1):27. doi:10.2754/avb201887010027 .
Radalj, Andrea, Nišavić, Jakov, Krnjaić, Dejan, Valčić, Miroslav, Jovanović, Tanja, Veljović, Ljubiša, Milić, Nenad, "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia" in Acta Veterinaria - Brno, 87, no. 1 (2018):27, https://doi.org/10.2754/avb201887010027 . .