Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses
2018
Authors
Ledina, TijanaMohar-Lorbeg, Petra
Golob, Majda
Đorđević, Jasna
Bogović-Matijasić, Bojana
Bulajić, Snežana
Article (Published version)
Metadata
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The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei... isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.
Keywords:
Antibiotic resistance / Tetracycline / Lactobacilli / Raw milk cheese / MALDI TOF mass spectrometry / Rep-PCRSource:
Journal of Food Science and Technology-Mysore, 2018, 55, 4, 1426-1434Publisher:
- Springer India, New Delhi
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1007/s13197-018-3057-6
ISSN: 0022-1155
PubMed: 29606757
WoS: 000428849100022
Scopus: 2-s2.0-85044647859
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Ledina, Tijana AU - Mohar-Lorbeg, Petra AU - Golob, Majda AU - Đorđević, Jasna AU - Bogović-Matijasić, Bojana AU - Bulajić, Snežana PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1635 AB - The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed. PB - Springer India, New Delhi T2 - Journal of Food Science and Technology-Mysore T1 - Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses VL - 55 IS - 4 SP - 1426 EP - 1434 DO - 10.1007/s13197-018-3057-6 ER -
@article{ author = "Ledina, Tijana and Mohar-Lorbeg, Petra and Golob, Majda and Đorđević, Jasna and Bogović-Matijasić, Bojana and Bulajić, Snežana", year = "2018", abstract = "The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.", publisher = "Springer India, New Delhi", journal = "Journal of Food Science and Technology-Mysore", title = "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses", volume = "55", number = "4", pages = "1426-1434", doi = "10.1007/s13197-018-3057-6" }
Ledina, T., Mohar-Lorbeg, P., Golob, M., Đorđević, J., Bogović-Matijasić, B.,& Bulajić, S.. (2018). Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore Springer India, New Delhi., 55(4), 1426-1434. https://doi.org/10.1007/s13197-018-3057-6
Ledina T, Mohar-Lorbeg P, Golob M, Đorđević J, Bogović-Matijasić B, Bulajić S. Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore. 2018;55(4):1426-1434. doi:10.1007/s13197-018-3057-6 .
Ledina, Tijana, Mohar-Lorbeg, Petra, Golob, Majda, Đorđević, Jasna, Bogović-Matijasić, Bojana, Bulajić, Snežana, "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses" in Journal of Food Science and Technology-Mysore, 55, no. 4 (2018):1426-1434, https://doi.org/10.1007/s13197-018-3057-6 . .