Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia
Abstract
The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phyloge...netic analysis of BoHV-1 isolates from Serbia.
Keywords:
BoHV-1 / phylogenetic analysisSource:
Revue De Medecine Veterinaire, 2018, 169, 7-9, 180-184Publisher:
- Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3
Funding / projects:
- Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens (RS-MESTD-Technological Development (TD or TR)-31008)
- Phylogenetic anaysis and molecular evolution of highly variable viruses: coinfections, host-pathogene interactions (RS-MESTD-Basic Research (BR or ON)-175024)
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Nišavić, Jakov AU - Knežević, Aleksandra AU - Stanojević, M. AU - Milić, Nenad AU - Radalj, Andrea PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1645 AB - The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia. PB - Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3 T2 - Revue De Medecine Veterinaire T1 - Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia VL - 169 IS - 7-9 SP - 180 EP - 184 UR - https://hdl.handle.net/21.15107/rcub_veterinar_1645 ER -
@article{ author = "Nišavić, Jakov and Knežević, Aleksandra and Stanojević, M. and Milić, Nenad and Radalj, Andrea", year = "2018", abstract = "The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.", publisher = "Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3", journal = "Revue De Medecine Veterinaire", title = "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia", volume = "169", number = "7-9", pages = "180-184", url = "https://hdl.handle.net/21.15107/rcub_veterinar_1645" }
Nišavić, J., Knežević, A., Stanojević, M., Milić, N.,& Radalj, A.. (2018). Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3., 169(7-9), 180-184. https://hdl.handle.net/21.15107/rcub_veterinar_1645
Nišavić J, Knežević A, Stanojević M, Milić N, Radalj A. Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire. 2018;169(7-9):180-184. https://hdl.handle.net/21.15107/rcub_veterinar_1645 .
Nišavić, Jakov, Knežević, Aleksandra, Stanojević, M., Milić, Nenad, Radalj, Andrea, "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia" in Revue De Medecine Veterinaire, 169, no. 7-9 (2018):180-184, https://hdl.handle.net/21.15107/rcub_veterinar_1645 .